Analysis of the function of CCL1 during respiratory tract infection in C-C motif ligand-1 transgenic mouse

C-C基序配体1转基因小鼠呼吸道感染过程中CCL1的功能分析

• 批准号: 23591111
• 负责人: INOUE Sumito
• 金额: $ 3.24万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591111/
• 关键词: 呼吸器; 感染症; 慢性閉塞性肺疾患; 遺伝子改変マウス; 呼吸器感染症; CCL1

We generated the CCL1 (NCBI: rs2282691) transgenic mice using surfactant protein C promoter (SPC-CCL1 Tg), which overexpressed CCL1 gene in the lungs. We developed the Mycobacterium bovis Bacillus Calmette Guérin (BCG) infection models in SPC-CCL1 Tg mice.Microscopic observation revealed that the numbers of granulomas in the lungs were significantly increased in SPC-CCL1 Tg mice. In DNA microarray analyses, 4,569 genes had 3 times and higher or one third and lower expression in the lung of Tg mice compare to that of WT mouse. Hierarchical cluster analysis of those genes revealed that Ern1, that plays important roles in endoplasmic reticulum stress and in granuloma formation, was more upregulated in the lung of BCG-treated SPC-CCL1 Tg mice compared to that of wild type mice.CCL1 overexpression increased the formation of granulomas upon BCG infection in the lungs. SPC-CCL1 Tg mice showed significant gene expression of Ern1 in comparison with wild type mice.

我们使用表面活性蛋白 C 启动子 (SPC-CCL1 Tg) 生成了 CCL1 (NCBI: rs2282691) 转基因小鼠，该启动子在肺部过度表达 CCL1 基因。我们在SPC-CCL1 Tg小鼠中建立了牛分枝杆菌卡介苗（BCG）感染模型。显微镜观察显示，SPC-CCL1 Tg小鼠肺部肉芽肿数量显着增加。在DNA微阵列分析中，与WT小鼠相比，Tg小鼠肺部中有4,569个基因的表达量是WT小鼠的3倍或更高或三分之一或更低。这些基因的层次聚类分析显示，与野生型小鼠相比，Ern1 在内质网应激和肉芽肿形成中起重要作用，在 BCG 治疗的 SPC-CCL1 Tg 小鼠的肺部中表达上调更多。CCL1 过度表达增加了 BCG 感染后肺部肉芽肿的形成。与野生型小鼠相比，SPC-CCL1 Tg 小鼠显示出显着的 Ern1 基因表达。

项目成果

Impairment of pulmonary function is an independent risk factor for atrial fibrillation: the Takahata study.

• DOI: 10.7150/ijms.8.514
• 发表时间: 2011
• 期刊: International journal of medical sciences
• 影响因子: 3.6
• 作者: Shibata Y;Watanabe T;Osaka D;Abe S;Inoue S;Tokairin Y;Igarashi A;Yamauchi K;Kimura T;Kishi H;Aida Y;Nunomiya K;Nemoto T;Sato M;Konta T;Kawata S;Kato T;Kayama T;Kubota I
• 通讯作者: Kubota I

Relationships between values of antibodies to several connective tissue disease autoantigens and pulmonary function in a Japanese general population: the Takahata study.

• DOI: 10.1371/journal.pone.0081678
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Nakano H;Shibata Y;Inoue S;Igarashi A;Yamauchi K;Abe S;Sato M;Aida Y;Nunomiya K;Kimura T;Nemoto T;Watanabe T;Konta T;Ueno Y;Kato T;Kayama T;Kubota I
• 通讯作者: Kubota I

Dominant negative(DN)MafBトランスジェニックマウスを用いた急性肺障害モデルの検討

使用显性阴性 (DN) MafB 转基因小鼠检查急性肺损伤模型

• 发表时间: 2012
• 作者: 會田康子;山内啓子;他
• 通讯作者: 他

A lower level of forced expiratory volume in 1 second is a risk factor for all-cause and cardiovascular mortality in a Japanese population: the Takahata study.

• DOI: 10.1371/journal.pone.0083725
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Shibata Y;Inoue S;Igarashi A;Yamauchi K;Abe S;Aida Y;Nunomiya K;Sato M;Nakano H;Sato K;Nemoto T;Kimura T;Watanabe T;Konta T;Daimon M;Ueno Y;Kato T;Kayama T;Kubota I
• 通讯作者: Kubota I

肺特異的CCL1高発現マウスにおける生体内細胞分画の検討

肺特异性 CCL1 高表达小鼠的体内细胞分级检查

• 发表时间: 2012
• 作者: Izuhara Y;Matsumoto H;Kanemitsu Y;Izuhara K;Tohda Y;Horiguchi T;Kita H;Kuwabara K;Tomii K;Otsuka K;Fujimura M;Ohkura N;Tomita K;Yokoyama A;Ohnishi H;Nakano Y;Oguma T;Hozawa S;Nagasaki T;Ito I;Oguma T;Inoue H;Tajiri T;Iwata T;Ono J,;岸宏幸,阿部修一,柴田陽光,五十嵐朗,山内啓子,佐藤正道,根本貴子,布宮慶子,會田康子,井上純人,久保田功
• 通讯作者: 岸宏幸,阿部修一,柴田陽光,五十嵐朗,山内啓子,佐藤正道,根本貴子,布宮慶子,會田康子,井上純人,久保田功