Regulation of reactive oxygen species induced by heavy-ion (carbon) beam; Pursuing the sophistication of heavy-ion cancer therapy

重离子（碳）束诱导的活性氧的调节；

• 批准号: 23591853
• 负责人: MATSUMOTO Ken-ichiro
• 金额: $ 3.24万
• 依托单位: National Institute of Radiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591853/
• 关键词: 重粒子線がん治療; 抗酸化剤; レドックス制御; 活性酸素; フリーラジカル; 放射線; 重粒子線; ヒドロキシルラジカル; スーパーオキサイド; 過酸化水素; 電子常磁性共鳴

Density of radiation induced hydroxyl radical could be measured by electron paramagnetic resonance (EPR) spin trapping method. Superoxide could be measured by EPR spin probing method. Hydrogen peroxide could also be measured by EPR spin probing method. Results from those measurements gave a prediction that the very dense hydroxyl radical generation by high LET radiation can not be regulated by biologically available antioxidants concentration. Other reactive oxygen species including low density hydroxyl radical could possibly be regulated or partly regulated using antioxidants.Inhibitory effect of TEMPOL to the radiation induced fibrosis caused by carbon-ion beam on mouse leg muscle was tested. Administration of TEMPOL dose affect to the fibrosis contrary to expectations.

用电子顺磁共振自旋捕获法可以测量辐射诱导的羟基自由基密度。超氧化物可以用EPR自旋探针法测量。过氧化氢也可以通过EPR自旋探针法测量。从这些测量的结果给出了一个预测，非常密集的羟基自由基产生高LET辐射不能由生物可利用的抗氧化剂浓度调节。抗氧化剂还可以调节或部分调节低密度羟基自由基等活性氧自由基的产生。TEMPOL对碳离子束所致小鼠腿部肌肉放射性纤维化有抑制作用。与预期相反，给予TEMPOL剂量对纤维化的影响。

项目成果

Density of Hydroxyl Radical Generated in an Aqueous Solution Irradiated by Carbon Ion Beam

碳离子束照射水溶液中产生的羟基自由基的密度

• DOI: 10.1016/j.freeradbiomed.2012.08.429
• 发表时间: 2012
• 期刊: Free Radical Biology & Medicine
• 影响因子: 7.4
• 作者: 吉田（橋立）智美;清水孝雄;中村元直;横山智史;松本謙一郎
• 通讯作者: 松本謙一郎