Inflammation in rat liver transplantation model

大鼠肝移植模型炎症反应

• 批准号: 23591881
• 负责人: ISHII Eiichi
• 金额: $ 3.33万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591881/
• 关键词: 移植; 肝臓移植; ラット; 拒絶反応; 抗体依存性拒絶反応; 細胞性拒絶反応; 肝動脈再建; 急性腎障害; 免疫寛容; 慢性拒絶反応; 免疫抑制剤; 虚血再灌流障害; 動脈再建

In the present study, we studied experimental orthotopic liver transplantation (OLT) model. We recognized that the Kamada technique and a new splint technique for hepatic artery reconstruction was convenient technique for rat OLT. We demonstrated that hepatic artery reconstruction prevented ischemic graft injury, inhibited graft rejection, and mediated long-term graft acceptance in rat OLT. In rejection of OLT, acute antibody-mediated rejection (AMR) clould develop in OLT and was characterized by endothelial cell injuries in microvasculature and by bile duct and hepatic cell degeneration and necrosis. In addition, chronic AMR could also involve in chronic rejection in OLT, and was characterized by typical late morphological changes with IgG deposition. In the development of tolerance in OLT, lymphangiogenesis may be involved in reduction of inflammation. Now we are studying the pathological characteristics of immune tolerance in rat OLT model.

本文对实验性原位肝移植(OLT)模型进行了研究。我们认为，Kamada技术和一种新的夹板技术重建肝动脉是一种方便的大鼠原位肝移植技术。在大鼠原位肝移植中，肝动脉重建预防了移植物缺血性损伤，抑制了移植物排斥反应，并介导了移植物的长期接受。急性抗体介导的排斥反应(AMR)是以微血管内皮细胞损伤、胆管和肝细胞变性、坏死为特征的急性抗体介导的排斥反应。此外，慢性AMR也可参与原位肝移植的慢性排斥反应，表现为典型的晚期形态学改变，并伴有免疫球蛋白沉积。在原位肝移植耐受的形成过程中，淋巴管生成可能参与了炎症反应的减轻。目前，我们正在研究大鼠原位肝移植模型免疫耐受的病理特点。