comprehensive breast cancer therapy targeting both estrogen receptor and Hedgehog signaling pathways

乳腺癌干细胞群的成瘤与增殖CD44 CD24-细胞高于CD44 CD24细胞。Hedgehog抑制剂处理显著抑制了这些增加。CD24 siRNA转染组细胞的侵袭力、克隆形成率、Shh和Gli1的表达均显著高于对照组。CD24 siRNA转基因细胞中ShH的抑制显著减少了肿瘤的发生和增殖，ERA通路在HH信号的激活中起着重要作用，提示ER通路参与了乳腺癌的进展。

Tumorigenesis and proliferation in breast canecr stem cell population; CD44+CD24- cells were higher than those in CD44+CD24+ cells. Hedgehog inhibitor treatment significantly abrogated these increases. Invasiveness, colony formation, and expressions of Shh and Gli1 in CD24 siRNA transfected cells were significantly higher than those in control. Shh suppression in CD24 siRNA transfected cells significantly reduced tumorigenesis and proliferation.ERa pathway plays an important role for Hh signaling activation, suggesting that ER pathway contributes to the progression in breast cancer.