Elucidation of tendon calcification inhibition mechanism of histamine H2-receptor antagonist

虽然以前有很多临床报道组胺H2受体拮抗剂（H2阻断剂）对钙化性肌腱炎和骨化分化有治疗作用，但关于其基础研究、作用机制的报道尚未见报道。本研究探讨了H2受体阻滞剂抑制肌腱钙化的基本机制。我们选择法莫替丁作为H2阻断剂，并将其应用于成骨细胞系MC 3 T3-E1和肌腱衍生细胞系TTD 6，证实法莫替丁在体外抑制钙化。接下来，我们证明了通过使用小鼠TTW，在体内跟腱中发生异位钙化，法莫替丁抑制异位钙化。法莫替丁对异位钙化的作用也在基础医学上得到证实。

Although there are many clinical reports that histamine H2-receptor antagonists (H2 blockers) is effective for the treatment of calcific tendinitis and ossification differentiation previously, there is no report about the basic research, the mechanism. We investigated the basic mechanisms of calcification inhibition of the tendon through the H2 blockers in this study. We selected the famotidine for H2 blocker and administrated to the osteoblast cell line MC3T3-E1 and tendon-derived cell lines TTD6 and conformed that famotidine suppressed the calcification in vitro. Next we demonstrated that by using a mouse TTW ,ectopic calcification occurs in the Achilles' tendon in vivo and famotidine suppressed the ectopic calcification. The effect of famotidine for ectopic calcification was also demonstrated on basic medicine.