Elucidation of tendon calcification inhibition mechanism of histamine H2-receptor antagonist

组胺H_2受体拮抗剂(H_2受体阻滞剂)治疗钙化性肌腱炎和骨化分化的临床报道虽多，但其基础研究、作用机制尚未见报道。在本研究中，我们探讨了H2受体阻滞剂抑制肌腱钙化的基本机制。我们选择法莫替丁作为H2阻断剂，分别作用于成骨细胞系MC3T3-E1和肌腱源性细胞系TTD6，证实法莫替丁对体外培养的TTD6细胞的钙化有抑制作用。接下来，我们通过使用小鼠TTW，在活体内证明了跟腱中发生了异位钙化，法莫替丁抑制了异位钙化。法莫替丁治疗异位钙化的效果也在基础医学上得到了证实。

Although there are many clinical reports that histamine H2-receptor antagonists (H2 blockers) is effective for the treatment of calcific tendinitis and ossification differentiation previously, there is no report about the basic research, the mechanism. We investigated the basic mechanisms of calcification inhibition of the tendon through the H2 blockers in this study. We selected the famotidine for H2 blocker and administrated to the osteoblast cell line MC3T3-E1 and tendon-derived cell lines TTD6 and conformed that famotidine suppressed the calcification in vitro. Next we demonstrated that by using a mouse TTW ,ectopic calcification occurs in the Achilles' tendon in vivo and famotidine suppressed the ectopic calcification. The effect of famotidine for ectopic calcification was also demonstrated on basic medicine.