The Inhibitory Effects of Intravenous Anesthetics on Substance P Receptor in U373 MG Human Astrocytoma Cells.

静脉麻醉药对 U373 MG 人星形细胞瘤细胞 P 物质受体的抑制作用。

• 批准号: 23592265
• 负责人: YAMAGUCHI Keisuke
• 金额: $ 3.24万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592265/
• 关键词: サブスタンスＰ; ケタミン; MAPK; 抗炎症作用; サイトカイン; サブスタンスＰ受容体; 悪性腫瘍; サブスタンスP受容体; 麻酔薬; ペントバルビタール; プロポフォール

The neuropeptide substance P (SP) is an important mediator of neurogenic inflammation within the central and peripheral nervous system. We investigated the effects of ketamine on NF-B phosphorylation, as well as the activation of the mitogen-activated protein kinase (MAPK) in the human astrocytoma cell line U373 MG cells which express high levels of SPR. The level of phosphorylated NF-kB and MAPK (ERK1/2, P-38, and JNK) were detected by western blotting. SP increased the phosphorylation of NF-kB and MAPKs and production of IL-6 and 8. Ketamine attenuated the phosphorylation of NF-kB p65 and MAPKs in U373 MG cells. Production of IL-6 and 8 were suppressed by ketamine. The inhibitory effects of ketamine on SP-induced cytokine production may be mediated by suppressing MAPKs via NF-kB activation. This finding suggests that the inhibition of SPR function by these compounds may be important in the analgesic effects of ketamine.

神经肽物质P（SP）是中央和周围神经系统内神经源性炎症的重要介体。我们研究了氯胺酮对NF-B磷酸化的影响，以及促分裂原激活蛋白激酶（MAPK）在人星形细细胞瘤细胞系U373 mg细胞中的激活，该细胞表达高水平的SPR。通过蛋白质印迹检测到磷酸化的NF-KB和MAPK（ERK1/2，P-38和JNK）的水平。 SP增加了NF-KB和MAPK的磷酸化以及IL-6和8的产生。氯胺酮减弱了U373 MG细胞中NF-KB P65和MAPK的磷酸化。 IL-6和8的产生被氯胺酮抑制。氯胺酮对SP诱导的细胞因子产生的抑制作用可以通过通过NF-KB激活抑制MAPK来介导。这一发现表明，这些化合物对SPR功能的抑制可能在氯胺酮的镇痛作用中很重要。

项目成果

Sevoflurane Suppresses TNF-α-Induced Inflammatory Responses in Pulmonary Epithelial Cells under Conditions of Anoxia/ Reoxygenation

七氟烷在缺氧/复氧条件下抑制 TNF-α 诱导的肺上皮细胞炎症反应

• 发表时间: 2013
• 期刊: Br J Anaesth
• 作者: Watanabe K;Iwabuchi K;Matsukawa T;Yokoyama Y;Yamaguchi K
• 通讯作者: Yamaguchi K

bitory effects of ketamine on substance P receptor in U373 MG human astrocytoma cells

氯胺酮对 U373 MG 人星形细胞瘤细胞 P 物质受体的咬合效应

• 发表时间: 2012
• 作者: Sugasawa Y;Yamaguchi K;Nagaoka I et al;Yamaguchi K
• 通讯作者: Yamaguchi K

Additive effect of sirolimus and anti-death receptor 5 agonistic antibody against hepatocellular carcinoma

西罗莫司与抗死亡受体5激动性抗体抗肝细胞癌的叠加作用

• 发表时间: 2013
• 期刊: Liver Int
• 影响因子: 6.7
• 作者: Kawahara T;Toso C;Yamaguchi K et al
• 通讯作者: Yamaguchi K et al

of Sevoflurane on Gene Expression in Pulmonary Epithelial Cells During Anoxia/Reoxygenation

七氟醚对缺氧/复氧期间肺上皮细胞基因表达的影响

• 发表时间: 2011
• 作者: Watanabe K;Iwahara C;Nakayama H;Iwabuchi K;Yamaguchi K;Kamiyama Y
• 通讯作者: Kamiyama Y

Effects of anesthesia with sevoflurane and propofol on the

七氟醚和异丙酚麻醉对患者的影响

• 发表时间: 2014
• 期刊: Int J Mol Med.
• 作者: Wakabayashi S;Yamaguchi K;Kumakura S;Murakami T;Someya A;Kajiyama Y,
• 通讯作者: Kajiyama Y,