The Inhibitory Effects of Intravenous Anesthetics on Substance P Receptor in U373 MG Human Astrocytoma Cells.

静脉麻醉药对 U373 MG 人星形细胞瘤细胞 P 物质受体的抑制作用。

• 批准号: 23592265
• 负责人: YAMAGUCHI Keisuke
• 金额: $ 3.24万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592265/
• 关键词: サブスタンスＰ; ケタミン; MAPK; 抗炎症作用; サイトカイン; サブスタンスＰ受容体; 悪性腫瘍; サブスタンスP受容体; 麻酔薬; ペントバルビタール; プロポフォール

The neuropeptide substance P (SP) is an important mediator of neurogenic inflammation within the central and peripheral nervous system. We investigated the effects of ketamine on NF-B phosphorylation, as well as the activation of the mitogen-activated protein kinase (MAPK) in the human astrocytoma cell line U373 MG cells which express high levels of SPR. The level of phosphorylated NF-kB and MAPK (ERK1/2, P-38, and JNK) were detected by western blotting. SP increased the phosphorylation of NF-kB and MAPKs and production of IL-6 and 8. Ketamine attenuated the phosphorylation of NF-kB p65 and MAPKs in U373 MG cells. Production of IL-6 and 8 were suppressed by ketamine. The inhibitory effects of ketamine on SP-induced cytokine production may be mediated by suppressing MAPKs via NF-kB activation. This finding suggests that the inhibition of SPR function by these compounds may be important in the analgesic effects of ketamine.

神经肽P物质（SP）是中枢和外周神经系统内神经源性炎症的重要介质。我们研究了氯胺酮对NF-B磷酸化的影响，以及在表达高水平SPR的人星形细胞瘤细胞系U373 MG细胞中丝裂原活化蛋白激酶（MAPK）的活化。Western blotting检测磷酸化NF-κ B和MAPK（ERK 1/2、P-38和JNK）的表达。SP增加NF-κ B和MAPK的磷酸化以及IL-6和IL-8的产生。氯胺酮可减弱U373 MG细胞NF-κ B p65和MAPK的磷酸化。氯胺酮可抑制IL-6和IL-8的产生。氯胺酮对SP诱导的细胞因子产生的抑制作用可能是通过NF-κ B激活抑制MAPK介导的。这一发现表明这些化合物对SPR功能的抑制在氯胺酮的镇痛作用中可能是重要的。

项目成果

Sevoflurane Suppresses TNF-α-Induced Inflammatory Responses in Pulmonary Epithelial Cells under Conditions of Anoxia/ Reoxygenation

七氟烷在缺氧/复氧条件下抑制 TNF-α 诱导的肺上皮细胞炎症反应

• 发表时间: 2013
• 期刊: Br J Anaesth
• 作者: Watanabe K;Iwabuchi K;Matsukawa T;Yokoyama Y;Yamaguchi K
• 通讯作者: Yamaguchi K

The effect of one-lung ventilation upon pulmonary inflammatory responses during lung resection

• DOI: 10.1007/s00540-011-1100-0
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF ANESTHESIA
• 影响因子: 2.8
• 作者: Sugasawa, Yusuke;Yamaguchi, Keisuke;Inada, Eiichi
• 通讯作者: Inada, Eiichi

bitory effects of ketamine on substance P receptor in U373 MG human astrocytoma cells

氯胺酮对 U373 MG 人星形细胞瘤细胞 P 物质受体的咬合效应

• 发表时间: 2012
• 作者: Sugasawa Y;Yamaguchi K;Nagaoka I et al;Yamaguchi K
• 通讯作者: Yamaguchi K

Effects of nitrous oxide on the production of cytokines and chemokines by the

一氧化二氮对细胞因子和趋化因子产生的影响

• 发表时间: 2013
• 期刊: Mol Med Rep.
• 作者: Kumakura S;Yamaguchi K;Sugasawa Y;Murakami T;Kikuchi T;Inada E;Nagaoka
• 通讯作者: Nagaoka

Antiemetic effect of naloxone in combination with dexamethasone and

纳洛酮联合地塞米松的止吐作用

• 发表时间: 2013
• 期刊: J Anesth.
• 作者: Kumakura S;Yamaguchi K;Sugasawa Y;Murakami T;Kikuchi T;Inada E;Nagaoka;Antiemetic effect of naloxone in combination with dexamethasone and
• 通讯作者: Antiemetic effect of naloxone in combination with dexamethasone and