Mechanisms of specific regulation of gonadotropn subunits expression by pulsatile GnRH stimulation

脉冲式 GnRH 刺激特异性调节促性腺激素亚基表达的机制

• 批准号: 23592404
• 负责人: KANASAKI Haruhiko
• 金额: $ 3.24万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592404/
• 关键词: GnRH; ゴナドトロピン; ＧｎＲＨ; TSA; FSH; PACAP

PACAP and its receptor are expressed not only in the central nervous system, but also in peripheral organs and function to stimulate pituitary hormone synthesis and release as both hypothalamic-pituitary releasing factor as well as an autocrine-paracrine factor within the pituitary. PACAP stimulates the expression of the gonadotropin alpha, LH betand FSH beta subunits as well as the GnRH receptor and its own PACAP type I receptor (PAC1R) in gonadotropin-secreting pituitary cells. In turn, GnRH, which has been known as a crucial component of gonadotropin secretion, stimulates the expression of PACAP and PAC1R in gonadotrophs. In addition, PAC1R presents in GnRH-producing neuronal cells and PACAP modulates the functions of GnRH neurons. It is evident that PACAP plays a role in reproductive function through regulating gonadotropins directly or indirectly by modulating GnRH release.

PACAP及其受体作为下丘脑-垂体释放因子和垂体内自分泌-旁分泌因子，不仅在中枢神经系统中表达，而且在外周器官中也有表达，具有刺激垂体激素合成和释放的功能。PACAP刺激促性腺激素分泌细胞的促性腺激素α、促黄体生成素β和促性腺激素释放激素β亚基以及促性腺激素释放激素受体及其自身的PACAP I型受体(PAC1R)的表达。GnRH被认为是促性腺激素分泌的关键成分，它反过来刺激促性腺激素细胞中PACAP和PAC1R的表达。此外，PAC1R存在于产生GnRH的神经细胞中，PACAP调节GnRH神经元的功能。显然，PACAP通过直接或间接地通过调节GnRH的释放来调节促性腺激素，从而发挥生殖功能。

项目成果

Prolonged stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells

促甲状腺激素释放激素和垂体腺苷酸环化酶激活多肽的长期刺激可使其在催乳素产生的 GH3 细胞中的受体功能脱敏

• 发表时间: 2013
• 期刊: Mol Cell Endocrinol
• 影响因子: 4.1
• 作者: Mijiddorj T;Kanasaki H;Sukhbaatar U;Oride A;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

Expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) potentiates the effects of GnRH on gonadotropin subunit gene expression.

• DOI: 10.1016/j.bbrc.2011.05.135
• 发表时间: 2011-07
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: I. Purwana;H. Kanasaki;A. Oride;T. Mijiddorj;K. Miyazaki

Possible role of PACAP and its PAC1 receptor in the differential regulation of pituitary LHβ- and FSHβ- subunit gene expression by pulsatile GnRH stimulation

PACAP 及其 PAC1 受体在脉动 GnRH 刺激对垂体 LHβ 和 FSHβ 亚基基因表达的差异调节中的可能作用

• 发表时间: 2013
• 期刊: Biol Reprod
• 影响因子: 3.6
• 作者: Kanasaki H;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

Follistatin, induced by thyrotropin-releasing hormone (TRH), plays no role in prolactin expression but affects gonadotropin FSHb expression as a paracrine factor in pituitary somatolactotroph GH3 cells

卵泡抑素由促甲状腺激素释放激素 (TRH) 诱导，在催乳素表达中不起任何作用，但会影响促性腺激素 FSHb 作为垂体促泌乳素细胞 GH3 细胞中的旁分泌因子的表达

• 发表时间: 2010
• 作者: Kanasaki H;Oride A;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

Extracellular Signal-Regulated Kinase (ERK) Activation and Mitogen-Activated Protein Kinase Phosphatase 1 Induction by Pulsatile Gonadotropin-Releasing Hormone in Pituitary Gonadotrophs.

• DOI: 10.1155/2012/198527
• 发表时间: 2012
• 期刊: Journal of signal transduction
• 作者: Kanasaki H;Purwana I;Oride A;Mijiddorj T;Miyazaki K
• 通讯作者: Miyazaki K