Relaxin and immune abnormality in human reproductive failure

本研究旨在探讨人松弛素对人滋养细胞来源的HTR-8/SV neo细胞株凋亡的影响，该细胞株可能是人上皮外滋养细胞（EVTs）的模型。用不同剂量的人重组（rH2）松弛素处理HTR-8/SV neo细胞。RXFP1和RXFP2 mRNA在HTR-8/SV neo细胞中表达。与未处理的对照组相比，rH2松弛素处理可降低HTR-8/SV neo细胞的tunel阳性率。Western blot分析显示，rH2松弛素降低了caspase-3和cleaved - PARP的表达，但增加了Bcl-2的表达。上述结果提示，rH2松弛素通过降低促凋亡caspase-3和cleaved PARP的表达，上调抗凋亡Bcl-2的表达，对HTR8/SV neo细胞具有抗凋亡作用。

The study was conducted to evaluate the effects of human relaxin on apoptosis in the human trophoblast derived HTR-8/SV neo cell line, which is a possible model of human extravillous trophoblasts (EVTs). HTR-8/SV neo cells were treated with different doses of human recombinant (rH2) relaxin. RXFP1 and RXFP2 mRNA expression was observed in HTR-8/SV neo cells. Compared with untreated control cultures, treatment with rH2 relaxin, decreased TUNEL-positive rate in HTR-8/SV neo cells was observed. Western blot analysis revealed that treatment with rH2 relaxin decreased the expression of caspase-3 and cleaved PARP, but in contrast increased Bcl-2 expression in those cells. These results suggest that rH2 relaxin has anti-apoptotic effects on HTR8/SV neo cells by decreasing pro-apoptotic caspase-3 and cleaved PARP expression and up-regulating anti-apoptotic Bcl-2 expression.