Identification of stem cell marker of mouse corneal epithelial cell

小鼠角膜上皮细胞干细胞标志物的鉴定

• 批准号: 23592607
• 负责人: SHIRAISHI Atsushi
• 金额: $ 3.24万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592607/
• 关键词: 再生医療; 角膜上皮; 形質転換; 再生医学; 眼科学; 幹細胞マーカー

We have established the mouse model for trans-differentiation from epidermal cell to corneal epithelial cell using K12-Cre-Zeg mouse in which green fluorescein protein (GFP) is expressed driven by Keratin 12 promoter. We first examined immunohistochemical staining, and results showed that K 10 was expressed in the center of the cornea and K10 and K12 were expressed in the limbus of the cornea in the trans-differentiation model. When side population cells (Sp cells) isolated from epidermis were co-cultured with cornel keratocyte isolated from corneal limbus, GFP positive cells were detected. RT-PCR results also showed positive for K 12. We then employed transcriptome analysis between the keratocytes isolated from central and limabal cornea. The results showed high expression of Wnt signal related mRNA were detected.

我们利用K12-Cre-Zeg小鼠建立了表皮细胞向角膜上皮细胞转分化的小鼠模型，在该模型中，由Keratin 12启动子驱动绿色荧光素蛋白（GFP）的表达。我们首先检测了免疫组化染色，结果显示在转分化模型中，K10在角膜中央表达，K10和K12在角膜的利姆布斯表达。将分离自表皮的侧群细胞（SP细胞）与分离自角膜利姆布斯的角膜基质细胞共培养，可检测到GFP阳性细胞。RT-PCR结果也显示K12阳性。 然后，我们在从中央角膜和角膜缘角膜分离的角膜细胞之间进行转录组分析。结果显示，Wnt信号相关mRNA在细胞中有高表达。

项目成果

Evaluation of Allergic Conjunctivitis by Thermography

• DOI: 10.1159/000357105
• 发表时间: 2014-01-01
• 期刊: OPHTHALMIC RESEARCH
• 影响因子: 2.1
• 作者: Hara, Yuko;Shiraishi, Atsushi;Ohashi, Yuichi
• 通讯作者: Ohashi, Yuichi

渦状角膜症における生体共焦点顕微鏡所見の検討

螺旋角膜病的生物共焦显微镜检查结果

• 发表时间: 2013
• 作者: 池川泰民;白石敦;大木元明義;大橋裕一
• 通讯作者: 大橋裕一

Diagnosis and clinical

诊断与临床

• 发表时间: 2011
• 期刊: Clin Experiment Ophthalmol.
• 作者: Hara Y;Shiraishi A;Yamaguchi M;Uno T;Ohashi Y.
• 通讯作者: Ohashi Y.

Use of 5-Cyano-2,3-Ditolyl-Tetrazolium Chloride Staining as an Indicator of Biocidal Activity in a Rapid Assay for Anti-Acanthamoeba Agents

• DOI: 10.1128/jcm.06461-11
• 发表时间: 2012-05-01
• 期刊: JOURNAL OF CLINICAL MICROBIOLOGY
• 影响因子: 9.4
• 作者: Kobayashi, Takeshi;Mito, Tsuyoshi;Ohashi, Yuichi
• 通讯作者: Ohashi, Yuichi

New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes

• DOI: 10.1186/1471-2415-13-47
• 发表时间: 2013-09-23
• 期刊: BMC OPHTHALMOLOGY
• 影响因子: 2
• 作者: Tasaka, Yoshitaka;Minami, Noriyoshi;Ohashi, Yuichi
• 通讯作者: Ohashi, Yuichi