myocardial interstitial serotonin and 5-hydroxyindole acetic acid levels during ischemia-reperfusion

应用微透析技术监测麻醉大鼠或家兔缺血再灌注时心肌间质5-羟色胺及其代谢物单胺氧化酶（MAO）、5-HIAA水平。在体外，缺血时5-HT水平升高，而5-HIAA水平下降。再灌注后5-羟色胺水平先升高后下降。再灌注后5-HIAA水平升高并保持高位。氟西汀降低缺血时5-HT水平，但对再灌注后5-HIAA水平无影响。Pargyline增加再灌注后5-HT水平，降低再灌注后5-HIAA水平。缺血时，载体介导的5-羟色胺外排可导致5-羟色胺水平升高，5-HIAA水平下降可归因于缺氧和酸中毒导致MAO活性降低。再灌注后，血液冲洗和摄取载体清除可导致5-HT水平下降。

Using microdialysis technique, we monitored myocardial interstitial 5-HT and its metabolite by monoamine oxidase (MAO), 5-HIAA levels in the ischemic region of anesthetized rat or rabbit during ischemia and reperfusion. In vehicle, 5-HT levels increased during ischemia, but 5-HIAA levels decreased during ischemia. After reperfusion 5-HT levels transiently increased and then declined. 5-HIAA levels increased after reperfusion and kept high level. Fluoxetine decreased 5-HT levels during ischemia, but did not change 5-HIAA levels after reperfusion. Pargyline increased 5-HT levels after reperfusion and decreased 5-HIAA levels after reperfusion. During ischemia, the carrier-mediated 5-HT efflux could contribute to the increase in 5-HT levels and the decrease in 5-HIAA levels could be ascribed to the reduction of MAO activity due to oxygen depletion and acidosis. After reperfusion, washout to blood as well as removal by uptake carrier could contribute to the decrease in 5-HT levels.