Abnormalities of Cardiac Sympathetic Nerve Ending Functions in Essential Hypertension

原发性高血压心脏交感神经末梢功能异常

• 批准号: 15590787
• 负责人: AKIYAMA Tsuyoshi
• 金额: $ 1.86万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590787/
• 关键词: hypertensive rat; sympathetic nerve endings; microdialysis technique; norepinephrine; dihydroxyphenylglycol; norepinephrine reuptke; 骨格筋交感神経終末; ノルエピネフリン分泌; 心臓交感神経終末; 星状神経節刺激

To elucidate whether sympathetic nerve ending functions are impaired in essential hypertension, we applied dialysis technique to the left ventricular myocardium of anesthetized SHR and WKY rats (20 weeks) and monitored myocardial interstitial norepinephrine(NE) and dihydroxyphenylglycol(DHPG) levels as indexes of NE release and intraneuronal NE metabolism on cardiac sympathetic nerve endings.There was no difference in heart rate between SHR and WKY, but the mean arterial pressure, the weight of wet heart, plasma NE concentration, and plasma DHPG concentration in SHR were higher than those in WKY.There was no difference in myocardial interstitial NE levels between SHR and WKY, but high K^+- and tyramine-induced interstitial NE responses in SHR were lower than those in WKY. These results suggest that NE release function on cardiac sympathetic nerve endings is impaired in SHR. There was no difference in myocardial interstitial DHPG levels and desipramine (NE reuptake inhibitor) -induced i … More nterstitial NE responses between SHR and WKY. These results suggest that NE reuptake and intraneuronal NE metabolic functions on cardiac sympathetic nerve endings are intact in SHR.Then, we focused on skeletal muscle sympathetic nerves innervating resistant arteries as causes of high plasma NE and DHPG levels. We applied dialysis technique to the left femoral skeletal muscle of anesthetized SHR and WKY rats (20 weeks) and monitored skeletal muscle interstitial NE and DHPG levels as indexes of NE release and intraneuronal NE metabolism on skeletal muscle sympathetic nerve endings.Skeletal muscle interstitial NE levels in SHR were higher than those in WKY. High K^+- and tyramine-induced interstitial NE responses in SHR were higher than those in WKY. These results suggest that NE release function on skeletal muscle sympathetic nerve endings is enhanced in SHR and this enhancement contributes to high plasma NE levels. Skeletal muscle interstitial DHPG levels in SHR were higher than WKY, but there was no difference in DHPG/NE ratios and desipramine-induced interstitial NE responses between SHR and WKY. These results suggest that NE reuptake and intraneuronal NE metabolic functions on skeletal muscle sympathetic nerve endings are intact in SHR. Less

To elucidate whether sympathetic nerve ending functions are impaired in essential hypertension, we applied dialysis technique to the left ventricular myocardium of anesthetized SHR and WKY rats (20 weeks) and monitored myocardial interstitial norepinephrine(NE) and dihydroxyphenylglycol(DHPG) levels as indexes of NE release and intraneuronal NE SHR和WKY之间心率没有差异，但SHR中的平均动脉压，湿心浓度和血浆DHPG浓度高于WKY中的平均动脉压，湿心的重量，血浆NE浓度和血浆DHPG浓度没有差异。 SHR和WKY之间的心肌间质NE水平没有差异，但是高的K^+ - SHR中的酪胺诱导的间质NE反应低于WKY中的SHR。这些结果表明，在SHR中，NE释放功能在心脏交感神经末端受到损害。心肌间质DHPG水平和去哌丁胺（NE再摄取抑制剂）诱导的I…SHR和WKY之间的更多内部性NE反应没有差异。这些结果表明，在SHR中，NE再摄取和神经内代谢作用在心脏交感神经末端的作用完好无损。然后，我们专注于骨骼肌交感神经，将抗性动脉支配为高血浆NE和DHPG水平的原因。我们将透析技术应用于麻醉的SHR和WKY大鼠的左股骨骨骼肌（20周），并监测了骨骼肌间质NE和DHPG水平，作为NE释放的索引和神经内神经内代谢的索引，在骨骼肌肉肌肉交感神经会上骨骼肌肉交感神经结束。 SHR中的高K^+ - 和酪胺诱导的间隙NE反应高于WKY。这些结果表明，SHR上的NE释放功能在骨骼肌交感神经末端有所增强，这种增强有助于高血浆NE水平。 SHR中的骨骼肌间隙DHPG水平高于WKY，但DHPG/NE的比率没有差异，而SHR和WKY之间的DHPG/NE比率和脱氨酰胺诱导的间质NE反应没有差异。这些结果表明，NE再摄取和神经内NE代谢功能在SHR中完好无损地对骨骼肌交感神经末端的作用完好无损。较少的

项目成果

Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate.

兔骨骼肌微透析液中 3-甲氧基-4-羟基苯基乙二醇的检测。

• 发表时间: 2003
• 期刊: J Chromatogr B Analyt Technol Biomed Life Sci 798
• 作者: Tokunaga N;Yamazaki T;Akiyama T;Mori H.
• 通讯作者: Mori H.

Tsuyoshi Akiyama: "Inhibition of cholinesterase elicits muscarinic receptor-mediated synaptic transmission in the rat adrenal medulla."Auton Neurosci. 107. 65-73 (2003)

Tsuyoshi Akiyama：“抑制胆碱酯酶会在大鼠肾上腺髓质中引发毒蕈碱受体介导的突触传递。”Auton Neurosci。

Extraneuronal enzymatic degradation of myocardial interstitial norepinephrine in the isohemic region

等血区心肌间质去甲肾上腺素的神经元外酶促降解

• 发表时间: 2004
• 期刊: Cardiovascular Research 64(1)
• 作者: Fujii T;Yamazaki T;Akiyama T;Sano S;Mori H.
• 通讯作者: Mori H.

Tsuyoshi Akiyama: "Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla."Neurochem Int. 44. 497-503 (2004)

Tsuyoshi Akiyama：“同时监测体内大鼠肾上腺髓质中乙酰胆碱和儿茶酚胺的释放。”Neurochem Int。

In vivo monitoring of norepinephrine and its metabolites in skeletal muscle.

骨骼肌中去甲肾上腺素及其代谢物的体内监测。

• 发表时间: 2003
• 期刊: Neurochem Int 43
• 作者: Tokunaga N;Yamazaki T;Akiyama T;Sano S;Mori H.
• 通讯作者: Mori H.