Role of Trps1 in temporomandibular development

Trps1 在颞下颌发育中的作用

• 批准号: 23592698
• 负责人: ABE Makoto
• 金额: $ 3.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592698/
• 关键词: 顎関節; 発生; 転写因子; TRPS1; ノックアウトマウス; 下顎; GATA; Trps1; コラーゲン; TRPS

Patients of Trichorhinophalangeal syndrome (TRPS) exhibit characteristic facial appearance which includes pear-shape nose and micrognathia (small jaw). In most of the case, TRPS results from mutation in the gene, TRPS1. In order to understand the mechanisms of the appearance of the facial phenotype in the TRPS patients, we examined the craniofacial development of the Trps1 knockout mice. The knockout mice were examined during its embryonic stage since this KO pups die soon after its birth. Trps1 was expressed throughout the embryonic stages of temporomandibular development. KO mice showed defects in mandibular bone development, especially in its proximal region. Articular disc, upper and lower joint cavities were never formed in the mutants. Also, the cellular proliferation at the condylar cartilage was significantly decreased.

毛鼻指关节综合征（TRPS）的患者表现出特征性的面部外观，包括梨形鼻和小颌。在大多数情况下，TRPS是由TRPS1基因突变引起的。为了了解TRPS患者面部表型出现的机制，我们检查了Trps1基因敲除小鼠的颅面发育。在其胚胎阶段检查敲除小鼠，因为该KO幼仔在其出生后不久死亡。Trps1在颞下颌发育的整个胚胎阶段都有表达。KO小鼠下颌骨发育缺陷，特别是在其近端区域。关节盘、上下关节腔在突变体中从未形成。同时，髁突软骨的细胞增殖也明显减少。

项目成果

Klf4は骨芽細胞間、ならびに細胞外基質との正常な接着を制御する

Klf4 控制成骨细胞之间及其与细胞外基质的正常粘附

• 发表时间: 2012
• 作者: 藤川順司;田中真理子;福士暁也;栗栖浩二郎;阿部真土
• 通讯作者: 阿部真土

Klf4 Regulates Osteoblast Differentiation

Klf4 调节成骨细胞分化

• 发表时间: 2011
• 作者: Michikami I;Abe M
• 通讯作者: Abe M

Activation of the Wnt/β-catenin pathway and tissue inhibitor of metalloprotease 1 during tertiary dentinogenesis.

• DOI: 10.1093/jb/mvs117
• 发表时间: 2013
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: Seisuke Yoshioka;Yusuke Takahashi;M. Abe;Ikumi Michikami;S. Imazato;S. Wakisaka;M. Hayashi;S. Ebisu

転写因子Klf4の骨格発生における役割解析

转录因子Klf4在骨骼发育中的作用分析

• 发表时间: 2013
• 作者: 藤川順司;田中真理子;森崎市治郎;阿部真土;脇坂聡
• 通讯作者: 脇坂聡

骨芽細胞でのKlf4の過剰発現は細胞外基質の発現を低下させ破骨細胞の分化を制御する

成骨细胞中 Klf4 的过度表达减少细胞外基质表达并控制破骨细胞分化

• 发表时间: 2012
• 作者: 田中真理子;藤川順次;伊藤祥作;阿部真土
• 通讯作者: 阿部真土