A research for new treatment with parathyroid hormone and cyclooxygenase-2 for mandibular bone invasion by oral squamous cell carcinoma

甲状旁腺激素和环氧合酶2治疗口腔鳞状细胞癌下颌骨侵犯的新方法研究

• 批准号: 23592945
• 负责人: SATOMI TAKAFUMI
• 金额: $ 3.24万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592945/
• 关键词: 口腔癌; 顎骨浸潤; PTH; COX-2

The aims of this study were to investigate the role of parathyroid hormone (PTH) up regulation by the cyclooxygenase-2 (COX-2) signaling pathway in bone invasion using oral squamous cell carcinoma (OSCC) mouse bone invasion model and to clarify new treatments of OSCC invasion into the jaw bone. We used 2 mouse SCC lines (NR-S1, SCC7) with different expressions of PTHrP and COX-2. C3H/HeN mice were inoculated with these cells in the masseter region to establish an animal model of mandibular invasion by OSCC. We measured tumor sizes of the mice, analyzed mandibular invasion by OSCC using micro-CT, then examined the TRAP staining and number of osteoclasts, and determined the mRNA expression of osteoclast-related cytokines by real-time PCR. We suggest that osteoclast-related cytokines play important roles in the bone invasion by OSCC cells.Consequently, PTHrP inhibition by COX-2 signaling is expected to be a therapeutic target to prevent bone invasion by OSCC cells.

本研究的目的是探讨环氧化酶-2（考克斯-2）信号通路上调甲状旁腺激素（PTH）在口腔鳞状细胞癌（OSCC）小鼠骨侵袭模型中的作用，并阐明OSCC侵袭颌骨的新治疗方法。我们使用了两种PTHrP和考克斯-2表达不同的小鼠SCC系（NR-S1，SCC 7）。将这些细胞接种于C3 H/HeN小鼠的咬肌区，以建立OSCC侵袭下颌骨的动物模型。我们测量了小鼠肿瘤的大小，使用micro-CT分析了OSCC对下颌骨的侵袭，然后检测了TRAP染色和破骨细胞的数量，并通过实时PCR测定了破骨细胞相关细胞因子的mRNA表达。我们认为破骨细胞相关的细胞因子在口腔鳞癌细胞的骨侵袭中起重要作用，因此，通过考克斯-2信号通路抑制PTHrP有望成为阻止口腔鳞癌细胞骨侵袭的治疗靶点。

项目成果

Solitary myofibroma of the mandible: an immunohistochemical and ultrastructural study with a review of the literature

下颌骨孤立性肌纤维瘤：免疫组织化学和超微结构研究及文献综述

• 发表时间: 2014
• 期刊: Med Mol Morphol
• 影响因子: 1.8
• 作者: Satomi T;Kohno M;Enomoto A;Abukawa H;Fujikawa K;Koizumi T;Chikazu D;Matsubayashi J;Nagao T
• 通讯作者: Nagao T

知っていますか?口の中のがんのこと〜口腔がんの予防と早期発見

您了解口腔癌的预防和早期发现吗？

• 发表时间: 2012
• 作者: Ogura N;Takahashi K;Iwai S;Fujimoto Y;Hashimoto H;Kondoh T;田所 晋;里見貴史;里見貴史
• 通讯作者: 里見貴史

Chondrosarcoma of the maxilla extending to the pterygomandibular space: A case report and review of the literature

上颌骨软骨肉瘤延伸至翼下颌间隙一例报告并文献复习

• 发表时间: 2012
• 期刊: J Maxillofac Oral Surg
• 作者: Satomi T;Kaneko T;Abukawa H;Hasegawa O;Watanabe M;Matsubayashi J;Nagao T;Chiba H;Chikazu D.
• 通讯作者: Chikazu D.

Effects of metabolic enzymes associated with fluoropyrimidine sensitivity in oral squamous cell carcinoma

口腔鳞状细胞癌中氟嘧啶敏感性相关代谢酶的影响

• 发表时间: 2011
• 作者: Masato Watanabe;Michihide Kohno;On Hasegawa;Harutsugi Abukawa;Takafumi Satomi;Tadayosi Kaneko;Akira Matsuo;Daichi Chikazu
• 通讯作者: Daichi Chikazu

Cyclo-Oxygenase-2 Expression Is Associated With Vascular Endothelial Growth Factor C Expression and Lymph Node Metastasis in Oral Squamous Cell Carcinoma

• DOI: 10.1016/j.joms.2013.04.015
• 发表时间: 2013-10-01
• 期刊: JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
• 影响因子: 1.9
• 作者: Kono, Michihide;Watanabe, Masato;Chikazu, Daichi
• 通讯作者: Chikazu, Daichi