Behavioral responses and Fos activation following orofacial formalin injection in a rodent model of Parkinson's disease

帕金森病啮齿动物模型口面部注射福尔马林后的行为反应和 Fos 激活

• 批准号: 23592988
• 负责人: NIWA Hitoshi
• 金额: $ 3.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592988/
• 关键词: パーキンソン病; 口腔顔面痛; 疼痛関連行動; ホルマリンテスト; 痛覚; c-Fos; ラット; 三叉神経; c－Fos; ﾎﾙﾏﾘﾝテスト

We investigated the modulation of the nociceptive stimuli from the orofacial region in Parkinson's disease (PD) model rats, which were produced by the unilateral or bilateral injection of 6-OHDA into the medial forebrain bundle. Behavioral responses to formalin injection into the upper lip and the expression of c-Fos and pERK in trigeminal spinal subnucleus caudalis (Vc) were explored.Unilateral PD rats showed increased nociceptive responses when formalin was injected ipsilaterally to the lesion. This hyperalgesic response was associated with increased c-Fos expression in Vc. When formalin was injected into contralateral side to 6-OHDA lesion, behavioral responses were reduced in 6-OHDA rats. However, the expression of pERK in Vc remained unchanged. Although the hyperalgesic response was not observed in bilateral PD rats, c-Fos expression in Vc increased.These results suggest that 6-OHDA resion causes hyperalgesic response to nociceptive stimulation from the orofacial region.

在帕金森病（PD）模型大鼠中，研究了单侧或双侧前脑内侧束注射6-OHDA对口面区伤害性刺激的调制作用。本实验观察了单侧PD大鼠上唇注射福尔马林后的行为反应及三叉神经脊束核尾侧亚核（Vc）内c-Fos和pERK的表达。这种痛觉过敏反应与Vc中c-Fos表达增加有关。对侧注射福尔马林后，6-OHDA大鼠的行为反应减弱。而pERK在Vc中的表达无明显变化。双侧PD大鼠虽然未观察到痛敏反应，但Vc中c-Fos表达增加，提示6-OHDA引起口面部伤害性刺激的痛敏反应。

项目成果

パーキンソン病モデルラットにおける顔面領域の疼痛刺激に対する行動学的反応とC-Fos発現

帕金森病模型大鼠面部疼痛刺激行为反应及 C-Fos 表达

• 发表时间: 2011
• 作者: 前川博治;朴 會士;正脇 綾;遠山 緑;丹羽 均
• 通讯作者: 丹羽 均