Elucidation of protein secreting mechanisms of filamentous fungi by the comparative genomic analysis

点击翻译按钮获取中文摘要

The cellulolytic filamentousfungus Trichoderma reesei secrets a tremendous amount of proteins and almost all of these proteins are cellulases and hemicellulases. Therefore, T. reesei is an ideal model organism to understand protein secretion mechanism of filamentous fungi. The goal of this study is to understand cellulase production mechanisms of T. reesei through comparative genomic analysis of a cellulase hyper-producing mutant lineage developed in Japan. After sequencing the genome of T. reesei mutants, by a Next-Generation Sequencer, a number of single nucleotide polymorphisms (SNPs) were identified. We focused on three mutated genes which are cre1 encoding the carbon catabolite repressor, bglr encoding transcriptional activator of intracellular beta-glucosidase, and bgl2 encoding major intracellular beta-glucosidase BGLII. From gene disruption and complementation of mutated nucleotide, these SNPs were found to be responsible for increased cellulase production.