Ca2+-nanodomain signaling: Development of techniques for its characterization and understanding its functional significance

Ca2-纳米域信号传导：开发其表征技术并了解其功能意义

• 批准号: 23650196
• 负责人: WADA Fumitaka
• 金额: $ 2.25万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650196/
• 关键词: 軸索; 成長円錐; ガイダンス; カルシウム; ミオシンVa; リアノジン受容体; VAMP2; エキソサイトーシス; カルシウムイオン; カルシウムシグナル; 国際情報交流

Neuronal circuits are formed by axons that have elongated and reached their targets. The tip of each axon, called the growth cone, explores extracellular guidance cues and can turn in the appropriated direction. In this project, we found that a Ca^<2+> nanodomain arising from an endoplasmic reticulum Ca^<2+> channel drives attractive guidance and that Myosin Va is released from the Ca^<2+> channel upon sensing the ^<2+> nanodomain leading to downstream signaling. We also showed that the dissociation of Myosin Va is sufficient for attractive growth cone turning.

神经元回路是由伸长并到达目标的轴突形成的。每个轴突的尖端，称为生长锥，探索细胞外的指导线索，并可以转向适当的方向。在这个项目中，我们发现来自内质网Ca^2+通道的Ca^2+纳米结构域驱动有吸引力的引导，并且肌球蛋白Va在感知到Ca ^2+纳米结构域后从Ca^2+通道释放，导致下游信号传导。我们还表明，肌球蛋白Va的解离足以吸引生长锥转向。