Comprehensive risk assessment of silver nanoparticles on fish molecular ecotoxicology

纳米银对鱼类分子生态毒理学的综合风险评估

• 批准号: 23651028
• 负责人: KASHIWADA Shosaku
• 金额: $ 2.66万
• 依托单位: Toyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651028/
• 关键词: 生態系影響評価; ナノマテリアル; メダカ; 銀ナノ粒子; 銀イオン; 生態リスク

We investigated silver nanocolloids (SNCs) effects on gene expression with DNA microarray analyses. 118 genes were up-regulated and 117 genes were down-regulated. Since severe morphological defects and damage of central nervous system were observed in SNCs-exposed medaka embryos, we chose five genes that were relevant to vertebrate embryogenesis and/or morphogenesis and well-studied in mammalian systems. Cathepsin L (ctsl), tropomyosin 1 alpha (tpm1), and retinol-binding protein (rbp) were significantly down-regulated in SNCs exposed embryos, while sarcoplasmic / endoplasmic reticulum calcium ATPase 1 (SERCA1, atp2a1) and homeobox B6b (hoxb6b) were both up-regulated. These genes were validated with with qRT-PCR. We performed gene knockdown techniques of RNAi. We observed the deformities in medaka embryos injected with siRNA of ctsl, tpm1 or rbp.We considered the three genes are target gene candidates of SNCs.

我们用DNA微阵列分析了银纳米胶体(SNCs)对基因表达的影响。118个基因表达上调，117个基因表达下调。由于SNCs暴露的青竹胚胎中观察到了严重的形态缺陷和中枢神经系统损伤，我们选择了5个与脊椎动物胚胎发生和/或形态发生相关的基因，并在哺乳动物系统中进行了充分的研究。组织蛋白酶L(CTSL)、原肌球蛋白1α(TPM1)和视黄醇结合蛋白(RBP)在SNCs暴露的胚胎中显著下调，而肌浆/内质网钙ATPase 1(SERCA1，atp2a1)和同源框B6b(Hoxb6b)均上调。用qRT-PCR对这些基因进行了验证。我们进行了RNAi的基因敲除技术。我们观察了注射CTs1、tpm1和rb3个siRNA的青竹胚胎的畸形，认为这三个基因是SNCs的靶基因候选。

项目成果

Salinity- and pH-dependent silver nano-toxicity in post-hatch embryos of Japanese medaka

日本青鳉孵化后胚胎的盐度和 pH 依赖性银纳米毒性

• 作者: Chisato Kataoka;Yuya Nakagame;Seiji Nagasaka;Hideo Kawaguchi;Shosaku Kashiwada
• 通讯作者: Shosaku Kashiwada

Salinity- dependent membrane permeation of silver nanocolloids in Japanese medaka egg embryos

日本青鳉卵胚胎中银纳米胶体的盐度依赖性膜渗透

• 作者: Tadashi Ariyoshi;Yuya Nakagame;Takuto Niwa;Chisato Kataoka;Hiroyuki Takei;Hiroshi Nakamura;Yoshihiro;Kagami;Shosaku Kashiwada
• 通讯作者: Shosaku Kashiwada

メダカ受精胚に対する銀ナノ粒子の毒性発現メカニズムの解明

阐明纳米银颗粒对青鳉受精胚胎的毒性机制

• 发表时间: 2013
• 作者: 丹羽拓人;中村 浩;鏡 良弘;柏田祥策
• 通讯作者: 柏田祥策

Stage-dependent toxicities of silver nanomaterials in early life stages of medaka

银纳米材料在青鳉生命早期阶段的阶段依赖性毒性

• 作者: Shosaku Kashiwada;Tadashi Ariyoshi;Takuto Niwa;Chisato Kataoka;Yuya Nakagame;Hiroyuki Takei
• 通讯作者: Hiroyuki Takei

魚類受精胚に対する銀イオンの毒性評価 ―ナノ物性工学を用いた新規試験法―

评估银离子对鱼类受精胚胎的毒性-利用纳米物理工程的新测试方法-

• 发表时间: 2011
• 作者: Matsuoka;S;白井信雄;仲亀雄哉，有吉理，丹羽拓人，長坂征治，竹井弘之，柏田祥策
• 通讯作者: 仲亀雄哉，有吉理，丹羽拓人，長坂征治，竹井弘之，柏田祥策