Mechanism of DNA damage responses mediated by acetylation of non-histone proteins

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We have newly identified several non-histone target proteins involved in DNA damage response, which are acetylated by histone acetyltransferases such as CBP and p300 in vitro. Among the identified acetylated proteins, acetylation of human Rad52 protein was induced after DNA double-strand break (DSB). We identified the acetylation sites of Rad52 by mass spectrometry with the in vitro- acetylated purified Rad52 protein. Radiation-induced homologous recombination was inhibited by mutations at the acetylated sites. Our results indicates that acetylation of Rad52 is required for DSB repair by homologous recombination.