Genome-wide analysis of chromatin structure at single molecule resolution by base modification and next-generation sequencing

通过碱基修饰和新一代测序以单分子分辨率对染色质结构进行全基因组分析

• 批准号: 23651199
• 负责人: ITO Takashi
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651199/
• 关键词: クロマチン構造; 次世代シーケンサ; 次世代シーケンス; クロマチン; 修飾酵素プロービング

To improve our understanding of chromatin dynamics, we have to reveal linkage/association among nucleosome/transcription factor?DNA interactions. Toward this goal, we tried to develop a novel method that utilizes base modifications, but not DNA cleavage, to reveal chromatin structure in a genome-wide scale. While a similar method termed NOME-Seq was reported, its combined use with PBAT, a bisulfite sequencing method of our own, would enable a practical and highly-sensitive analysis.

为了提高我们对染色质动力学的理解，我们必须揭示核小体/转录因子之间的连锁/关联？DNA相互作用为了实现这一目标，我们试图开发一种新方法，该方法利用碱基修饰而不是DNA切割来揭示全基因组范围内的染色质结构。虽然报道了称为NOME-Seq的类似方法，但其与PBAT（我们自己的亚硫酸氢盐测序方法）的组合使用将实现实用且高度灵敏的分析。

项目成果

Amplification-free whole-genome bisulfite sequencing by post-bisulite adaptor tagging

通过亚硫酸氢盐后接头标记进行无扩增全基因组亚硫酸氢盐测序

• 发表时间: 2012
• 期刊: Nucleic Acids Res
• 影响因子: 14.9
• 作者: Fumihito Miura;Yusuke Enomoto;Ryo Dairiki and Takashi Ito
• 通讯作者: Ryo Dairiki and Takashi Ito