A CHARENGE FOR CRYOPRESERVATION OF FISH EGGS WITH LIQUID MENISCUS

为了减小鱼卵冷冻过程中外部结冰所引起的机械应力，提出了一种利用液体弯月面的新方法。我们已经开发了弯月面保持器，该保持器将液体弯月面保持到鱼卵的顶部。鱼卵的膜被海藻糖水溶液的薄膜包裹，可以保护鱼卵免受外部结冰的影响。此外，我们使用脱水冷冻，以减少破裂，由于内部结冰。实验结果表明，带弯月形保持器的鱼卵解冻后具有较高的保形率。

In order to reduce mechanical stress induced by external ice formation in a freezing of fish egg, we suggest a new method using liquid meniscus. We have developed the meniscus holder that holds the liquid-meniscus up to the top of the fish egg. The membrane of the fish egg is coated by the thin film of torehalose aqueous and can be protected from the external ice formation. Also, we used the dehydro-freezing in order to reduce the rupture due to the internal ice formation. The experimental results show that the fish eggs with meniscus holder have a high shape-preservation rate after the thawing.