A CHARENGE FOR CRYOPRESERVATION OF FISH EGGS WITH LIQUID MENISCUS

为了减少鱼卵冷冻过程中外部结冰引起的机械应力，我们提出了一种使用液体半月板的新方法。我们开发了半月板支架，可以将液体半月板固定在鱼卵的顶部。鱼卵的膜被海藻糖水薄膜覆盖，可以防止外部结冰。此外，我们使用脱氢冷冻，以减少破裂，由于内部冰的形成。实验结果表明，带半月板支架的鱼卵在解冻后具有较高的形状保存率。

In order to reduce mechanical stress induced by external ice formation in a freezing of fish egg, we suggest a new method using liquid meniscus. We have developed the meniscus holder that holds the liquid-meniscus up to the top of the fish egg. The membrane of the fish egg is coated by the thin film of torehalose aqueous and can be protected from the external ice formation. Also, we used the dehydro-freezing in order to reduce the rupture due to the internal ice formation. The experimental results show that the fish eggs with meniscus holder have a high shape-preservation rate after the thawing.