Investigation of kinetic change and gene expression of yeast cells in local nanosecond pulsed electric field

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In the present study, we tried to apply a nanosecond pulsed electric field to the yeastcells precisely arranged in the micro-reactor and to measure their behavior variation. We also verified the correlation between gene expression and local instantaneous fieldon the basis of both analyses of gene amplification and cell model.As a major achievement, the pulse power supply with precise control of the waveform wasdeveloped successfully. In addition, we identified the variable range of pulse width and the optimum condition for dispersion and sequence of standard particles. The dependence of membrane damage degree and respiratory activity on stress change was classified by nucleic acid staining state. Moreover, the variation of electrical properties due tomembrane modification to was quantified using numerical model of cell structure, and themain factor gene was verified by polymerase chain reaction analysis.