Investigation of the dielectrophoretic properties of parasitic bacteria in emulsion and construction of new separation and detection systems of bacteria

本研究对乳状液中寄生细菌的介电性质进行了研究，并研制了一种新的分离检测装置，用于快速、简便的检测。通过对微细胞内电场分布、细菌电泳速和电泳动轨迹的数值分析，得到了最佳的电极布置和油滴分离条件。在实际应用中，实现了对各种细菌的选择性缩合。采用微孔滤膜和LCR仪相结合的方法对细菌数量进行了定量估算。

In the present study, the dielectrophoretic properties of parasitic bacteria in emulsion were investigated, and new separation and measurement apparatus was developed for rapid and simple detection. Optimum electrode arrangement and separation condition from oil droplet were obtained by numerical analyses of electric field distribution in microcell, and dielectrophoretic velocity and trajectory of bacteria. In actual, selective condensation was achieved for various kinds of bacteria. Moreover, the bacterial amount was estimated quantitatively by combination of dielectrophoretic micro filter and LCR meter.