The supercooling technology in preservation of organ grafts

过冷技术在器官移植保存中的应用

• 批准号: 23791511
• 负责人: WATANABE Toshihiko
• 金额: $ 2.75万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791511/
• 关键词: 過冷却凍結; 臓器保存; 臓器移植; 心移植; 脳死移植; 磁場環境下凍結; マウス

Current medical transplantation confronts major problems such as the shortage of donors and geographical restrictions that inhibit efficient utilization of finite donor organs. We investigated whether a strategy which best preserves organ grafts can be achieved by the use of a novel refrigerating chamber, which is capable of establishing a supercooled and unfrozen state stably by generating an electrostatic field in its inside. When mice heart was stored in the supercooled conditions, the levels of major biochemical markers leaked from the preserved organs were significantly lower than in the ordinary hypothermic storage. No apparent tissue damages were observed histologically after the supercooled preservation. After heart transplantation, rate of re-heart beating in supercooled condition was significantly higher. Our results suggest that the use of this supercooling refrigerator improves organ preservation and may provide an innovative technique for human organ transplantation.

目前的医学移植面临的主要问题，如供体短缺和地理限制，抑制了有限的供体器官的有效利用。我们研究了是否可以通过使用一种新型的冷藏室来实现最好地保存器官移植物的策略，该冷藏室能够通过在其内部产生静电场来稳定地建立过冷和解冻状态。小鼠心脏在过冷条件下保存时，其主要生化指标的漏出量明显低于普通低温保存。过冷保存后，组织学上未观察到明显的组织损伤。心脏移植术后，过冷状态下心脏复跳率明显增高。我们的研究结果表明，使用这种过冷冰箱提高了器官保存，并可能为人体器官移植提供一种创新技术。

项目成果

Congenital median raphe cysts : Coexistence of cystic lesions and canal like lesions

先天性中缝囊肿：囊性病变和管样病变并存

• 发表时间: 2013
• 期刊: Open J Pediatr 2013
• 作者: Takahashi M;Watanabe T;Sato K;Ohno M;Yamada K;Takezoe T;Fuchimoto Y;Ohkita H;Matsuoka K;Kanamori Y.
• 通讯作者: Kanamori Y.

Congenital pouch colonの1例

先天性袋状结肠的一个例子

• 发表时间: 2013
• 作者: 渡辺稔彦;大野通暢;渕本康史
• 通讯作者: 渕本康史

Cecal volvulus associated with mobile cecum in Cornelia de Lange syndrome : Report of a case

科妮莉亚·德·朗格综合征中与盲肠移动相关的盲肠扭转：一例报告

• DOI: 10.4236/ojped.2013.32018
• 发表时间: 2013
• 期刊: Open J Pediatr 2013
• 作者: Takahashi M;Watanabe T;Tanaka H;Yamada W;Yamada K;Fuchimoto Y;Nosaka S;Kanamori Y.
• 通讯作者: Kanamori Y.

肺葉外肺分画症に対して胸腔鏡下手術を施行した2例

胸腔镜肺叶外部分手术二例

• 发表时间: 2013
• 作者: 渡辺稔彦;清水隆弘;高橋正貴;大野通暢;佐藤かおり;山田耕嗣;竹添豊志子;樋口昌孝;川崎一輝;渕本康史;金森豊
• 通讯作者: 金森豊

Incidence and characteristics of contralateral manifestation in children with unilateral inguinal hernia after laparoscopic completely extraperitoneal repair

小儿单侧腹股沟疝腹腔镜完全腹膜外修补术后对侧表现的发生率及特点

• 发表时间: 2013
• 作者: Toshihiko Watanabe;Fumiko Yoshida;Masaharu Mori;Etsuji Ukiyama;Miwako Nakano;Masao Endo
• 通讯作者: Masao Endo