The development of chemohypethermia using magnetoliposomes in magnetic field

在以前的研究中，我们研究了电磁加热与磁铁矿负载的脂质体在癌症治疗中的轻度消融的目的。本研究的主要目的是确定是否可以通过用磁铁将我们的含有磁铁矿的脂质体吸引到肿瘤来增强载磁铁矿脂质体的治疗功效。通过感应加热处理具有模拟人食管癌的TE-8细胞的裸鼠。为了探讨这种方法对肿瘤生长的影响，将裸小鼠分为3个实验组：加热组（磁铁）、加热组（无磁铁）和对照组。在加热组（磁体）中，用磁体将装载有磁铁矿的脂质体吸引到肿瘤，并且在装载有磁铁矿的脂质体注射后将荷瘤小鼠置于交变磁场（AMF）下10分钟，而在加热组（无磁体）中，在不存在磁体的情况下将装载有磁铁矿的脂质体注射到每只动物中，并且施用AMF治疗。肿瘤体积在对照组和其他组之间有显著差异。此外，在加热组（磁体）中，在治疗后3天，可以在肿瘤中清楚地检测到负载有磁铁矿的脂质体。因此，这种新的消融方法可能是一种可行的癌症治疗技术。

In a previous study, we investigated electromagnetic heating with magnetite-loaded liposomes for the purpose of mild ablation in cancer treatment. The principal aim of this study was to determine whether the therapeutic efficacy of magnetite-loaded liposomes could be enhanced by attracting our liposomes containing magnetite to the tumor with magnet. Nude mice with TE-8 cells mimicking human esophageal cancer were treated by induction heating. To investigate the influence of this method on tumor growth, nude mice were assigned to 3 experimental groups: heating group (magnet), heating group (no magnet), and control group. In the heating group (magnet), magnetite-loaded liposomes were attracted to the tumor with a magnet and tumor-bearing mice placed for 10 min under an alternating magnetic field (AMF) after magnetite-loaded liposomes injection, while in the heating group (no magnet), magnetite-loaded liposomes were injected into each animal in absence of magnet and AMF treatment was administered. Tumor volume was significantly different between the control group and the other groups. Furthermore, the magnetite-loaded liposomes could be detected clearly in the tumor 3 days after treatment in heating groups (magnet). Thus, this new ablation method may be a feasible technique for cancer therapy.