Establishementof whole ovarian cryopreservation

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The objective of this study is toestablish a technology for freezingovarian tissues using primates (cynomolgus monkeys). A technique to remove ovaries with the ovarian arteries and veins was examined in cynomolgus monkeys. Conditions of cryoprotectants used inovary freezing as well as those of slow freezing methods were studied. To evaluatethe function of frozen-thawed ovaries, viability of the cells was measured using propidium iodide (PI) and the level of estrogen in the culture solution after adding FSH was measured by ELISA.