Establishementof whole ovarian cryopreservation

全卵巢冷冻保存的建立

• 批准号: 23791831
• 负责人: SHIMIZU Yoshihiko
• 金额: $ 2.75万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791831/
• 关键词: 生殖医学; 卵巣凍結

The objective of this study is toestablish a technology for freezingovarian tissues using primates (cynomolgus monkeys). A technique to remove ovaries with the ovarian arteries and veins was examined in cynomolgus monkeys. Conditions of cryoprotectants used inovary freezing as well as those of slow freezing methods were studied. To evaluatethe function of frozen-thawed ovaries, viability of the cells was measured using propidium iodide (PI) and the level of estrogen in the culture solution after adding FSH was measured by ELISA.

本研究的目的是建立一种灵长类动物（食蟹猴）卵巢组织的冷冻技术。在食蟹猴中检查了用卵巢动脉和静脉切除卵巢的技术。研究了低温冷冻和慢速冷冻时保护剂的使用条件。用碘化丙啶（PI）法测定卵巢细胞活力，ELISA法测定加入FSH后培养液中雌激素水平，评价卵巢冻融后的功能。

项目成果

生児を得ることができた macrocephalic sperm head syndrome の 2 例

巨头型精子头综合征导致活产2例

• 发表时间: 2012
• 作者: 清水良彦;伊津野美香;金子由貴;岸田和美;段亜儒;竹林明枝;高島明子;木村文則;喜多伸幸;高橋健太郎;村上節
• 通讯作者: 村上節

Successful delivery following ICSI with macrocephalic sperm head syndrome: a case report.

巨头精子头综合征 ICSI 后成功分娩：病例报告。

• DOI: 10.1016/j.rbmo.2012.02.022
• 发表时间: 2012
• 期刊: Reprod Biomed Online.
• 作者: Shimizu Y;Kiumura F;Kaku S;Izuno M;Tomita K;Thumkeo D;Murakami T.
• 通讯作者: Murakami T.

円錐切除後に頸管狭窄をきたした挙児希望症例の管理～狭窄の解除と子宮内膜炎の治療を行い体外受精で妊娠に至った1例

锥切术后因宫颈狭窄而想要生育的患者的处理 - 切除狭窄并治疗子宫内膜炎，通过体外受精怀孕的案例

• 作者: Ikeda K;Higashi T;Sugawara K;Tomori K;Kinoshita H;Kasai T;髙橋健太郎
• 通讯作者: 髙橋健太郎

Intake of Vinegar Beverage Is Associated with Restoration of Ovulatory Function in Women with Polycystic Ovary Syndrome

醋饮料的摄入与多囊卵巢综合症女性排卵功能的恢复有关

• 发表时间: 2013
• 期刊: Tohoku J. Exp. Med.
• 作者: Di Wu;Fuminori Kimura;Akiko Takashima-Murata;Yoshihiko Shimizu;Akie Takebayahi-Shiraga;Nobuyuki Kita;GuangMei Zhang and Takashi Murakami
• 通讯作者: GuangMei Zhang and Takashi Murakami

Comparison of the outcome of in vitro fertilization after laparoscopic laser ablation surgery versus laparoscopic cystectomy for endometrioma

腹腔镜激光消融术与腹腔镜子宫内膜异位囊肿切除术后体外受精结果的比较

• DOI: 10.1016/j.gmit.2012.12.005
• 发表时间: 2013
• 期刊: Gynecology and minimally invasive therapy
• 作者: Akie Takebayashi;Shimizu Y;Akimasa Takahashi;Akiyoshi Yamanaka;Akiko Takashima;Fuminori Kiumura;Nobuyuki Kita;Kentaro Takahashi;Takashi Murakami.
• 通讯作者: Takashi Murakami.