Development of novel therapeutics to inhibit cell proliferation viaPOU6F1 in ovarian clear cell carcinoma

ARID1A 是 SWI/SNF 染色质重塑复合体的成员，其成员具有螺旋和 ATP 酶活性，被认为通过改变某些基因周围的染色质结构来调节某些基因的转录。 ARID1A（一种假定的肿瘤抑制因子）蛋白 BAF250a 的缺失最近被描述为卵巢透明细胞癌中的常见事件。在这项研究中，我们报告恢复含有 ARID1A 突变的卵巢透明细胞癌细胞中野生型 ARID1A 的表达足以抑制细胞增殖并增强抗癌药物的敏感性。基因表达分析确定了 ARID1A 的几个下游靶标。我们的结果提供了功能证据来支持 ARID1A 是真正的肿瘤抑制基因的假设。

ARID1A, a member of SWI/SNF chromatin remodeling complex, whose members have helices and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Loss of the ARID1A (a putative tumor suppressor) protein BAF250a has recently been described as a frequent event in ovarian clear cell carcinoma. In this study, we report that restoring wild-type ARID1A expression in ovarian clear cell carcinoma cells that harbor ARID1A mutations is sufficient to suppress cell proliferation and enhance anticancer agent sensitivity. Gene expression analysis identified several down stream targets of ARID1A. Our results provide functional evidence in support of the hypothesis that ARID1A is a bona fide tumor suppressor gene.