Quantitative analysis of fundus autofluorescence based on an excitation wavelength of 532nm.

用日本爱知NIDEK公司生产的F-10型激光扫描检眼镜采集激发波长为532 nm(532AF)的眼底自发荧光图像。分析532AF在正常眼和病理眼中的表现。1)在正常眼中，532AF的荧光亮度分布与490 nm激发光(490AF)的自发荧光相似。由于532 nm光仅被黄斑色素轻微阻挡，532AF可以很好地观察到黄斑中心凹的自发荧光。2)中心性浆液性脉络膜视网膜病眼可见532AF异常表现。在浆液性视网膜脱离(SRD)区观察到Hyper-532AF，并在视网膜外损害处SRD消退。视网膜色素上皮损伤区可见Hypo-532AF。3)在改装后的F-10内安装了参比荧光探针，用于532AF的定量分析。对532AF眼底和参照头的亮度进行对比分析。这些数据目前正在调查中。

Fundus autofluorescence images based on an excitation wavelength of 532nm (532AF) were acquired using modified scanning laser ophthalmoscope: F-10 (NIDEK, Aichi, Japan). The findings of 532AF in normal eyes and pathologic eyes were analyzed. 1) In normal eyes, fluorescent brightness distribution of 532AF was similar to that of autofluorescence based on 490nm excitation light (490AF). As 532nm light is only slightly blocked by macular pigments, foveal autofluorescence was well observed by 532AF. 2) Abnormal findings of 532AF were observed in eyes with central serous chorioretinopathy. Hyper-532AF was observed in the area with serous retinal detachment (SRD) and resolved SRD where outer retina was damaged. Hypo-532AF was seen in the area with damaged retinal pigment epithelium. 3) Reference fluorescent tip was installed inside of the modified F-10 for quantitative analysis of 532AF. 532AF brightness of both fundus and reference tip were compared and analyzed. The data were under investigation now.