The generation of ameloblasts from adult tissue-delivered cells and the method of the tooth germ regenration

从成体组织递送细胞产生成釉细胞和牙胚再生方法

• 批准号: 23792176
• 负责人: SUZUKI Shigeki
• 金额: $ 2.66万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792176/
• 关键词: 象牙芽細胞; DMP-1; DSPP; 保存修復; 口腔粘膜上皮

The original main purpose of this research project was the identification of the factors that induce epithelial-mesenchymal interaction during tooth development. However, it was difficult to isolate and cultivate oral epithelial cells. Since it has been suggested that the enamel and dentin extracellular matrix (ECM) proteins would be able to regulate ameloblasts and odontoblasts differentiation in vitro, we generated the recombinant proteins of tooth ECM proteins and analyzed their effects for mesenchymal stem cells (MSCs) adhesion and differentiation. DMP-1 was able to induce MSCs adhesion and differentiation into osteogenic lineage cells while intact DPP was unable to. Thus, the functions of these proteins may be modulated by proteolytic cleavages by unknown extracellular proteases.

本研究项目最初的主要目的是鉴定在牙齿发育过程中诱导上皮-间充质相互作用的因素。然而，口腔上皮细胞的分离和培养是困难的。由于已经提出，釉质和牙本质细胞外基质（ECM）蛋白将能够调节成釉细胞和成牙本质细胞的分化在体外，我们产生的重组蛋白质的牙齿ECM蛋白，并分析其对间充质干细胞（MSCs）的粘附和分化的影响。BMP-1能够诱导MSCs粘附并分化为成骨细胞，而完整的DPP不能。因此，这些蛋白质的功能可以通过未知的细胞外蛋白酶的蛋白水解裂解来调节。

项目成果

Repeat sequence 改変型およびRGD配列改変型 Dentin sialophosphoprotein発現株の作製

重复序列修饰型和RGD序列修饰型牙本质唾液酸磷蛋白表达株的制备

• 发表时间: 2012
• 作者: 小武家 誠司;鈴木 茂樹;西村 英紀
• 通讯作者: 西村 英紀

Lessons Learned from Mouse Models of DSPP, DSP, and DPP

DSPP、DSP 和 DPP 鼠标模型的经验教训

• 发表时间: 2012
• 期刊: Bentham Science Publishers
• 作者: Suzuki;S.;and Kulkarni;A. B.
• 通讯作者: A. B.

Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues.

• DOI: 10.1016/j.archoralbio.2012.03.005
• 发表时间: 2012-09
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Suzuki, Shigeki;Haruyama, Naoto;Nishimura, Fusanori;Kulkarni, Ashok B.
• 通讯作者: Kulkarni, Ashok B.

内在性Wntによる歯髄創傷治癒促進効果とDentin Phosphoproteinによる硬組織形成作用の検討

内源性Wnt促进牙髓创面愈合及牙本质磷蛋白硬组织形成作用的考察

• 发表时间: 2013
• 作者: Yoshiba N;Yoshiba K;Ohkura N;Shigetani Y;Takei E;Hosoya A;Nakamura H;Okiji T;鈴木茂樹
• 通讯作者: 鈴木茂樹

The inflammation-lipocalin 2 axis may contribute to the development of chronic kidney disease

• DOI: 10.1093/ndt/gft449
• 发表时间: 2014-03-01
• 期刊: NEPHROLOGY DIALYSIS TRANSPLANTATION
• 影响因子: 6.1
• 作者: Hashikata, Atsushi;Yamashita, Akiko;Nishimura, Fusanori
• 通讯作者: Nishimura, Fusanori