The elucidation of mechanisms and significance of DSPP cleavage in dentiongenesis

DSPP 裂解在牙本质形成中的机制和意义的阐明

• 批准号: 21890158
• 负责人: SUZUKI Shigeki
• 金额: $ 1.66万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890158/
• 关键词: 象牙質; DSPP; 遺伝子改変マウス; 過剰発現細胞株

Dentin phosphoprotein (DPP) and dentin sialoprotein (DSP) are cleaved products of precursor dentin sialophosphoprotein (DSPP). In this research, we explored the mechanisms and the biological significance of DSPP cleavage into two proteins using cell lines expressing cleavage-resistant DSPP. We observed DSP specific band under 100kDa from the condition medium of normal DSPP transfectants. Conversely, we detected DSP and DPP specific bands over 100kDa from mutated DSPP transfectants. Interestingly, the intensity of DSP and DPP specific bands of mutated DSPP was apparently higher than that of normal DSPP. These results suggested that we successfully generated stable trasnfectants expressing normal or mutated DSPP and cleavage of DSPP might affect the secretion or the extracellular deposition of DSP and DPP.

牙本质磷蛋白（DPP）和牙本质唾液蛋白（DSP）是前体牙本质唾液磷蛋白（DSPP）的裂解产物。在这项研究中，我们利用表达抗裂DSPP的细胞系，探讨了DSPP分裂成两种蛋白的机制和生物学意义。在正常DSPP转染的条件培养基中，在100kDa下观察到DSP特异带。相反，我们从突变的DSPP转染物中检测到超过100kDa的DSP和DPP特异性条带。有趣的是，突变DSPP的DSP和DPP特异性条带的强度明显高于正常DSPP。这些结果表明，我们成功制备了表达正常或突变DSPP的稳定转染物，DSPP的裂解可能影响DSP和DPP的分泌或胞外沉积。

项目成果

A method for rapid demineralization of teeth and bones.

• DOI: 10.2174/1874210601004010223
• 发表时间: 2010-12-15
• 期刊: The open dentistry journal
• 作者: Cho A;Suzuki S;Hatakeyama J;Haruyama N;Kulkarni AB
• 通讯作者: Kulkarni AB

The Estabhshment of Stable Cell Lines Expressing Cleavage-resistant Dentin Sialophosphoprotein

表达抗裂解牙本质唾液酸磷蛋白的稳定细胞系的建立

• 发表时间: 2011
• 作者: Hatakeyama;J.;Fukumoto;S.;et al.;Morita K;Shigeki Suzuki
• 通讯作者: Shigeki Suzuki

Dentin sialoprotein and dentin phosphoprotein have distinct roles in dentin mineralization.

• DOI: 10.1016/j.matbio.2009.03.006
• 发表时间: 2009-05
• 期刊: MATRIX BIOLOGY
• 影响因子: 6.9
• 作者: Suzuki, Shigeki;Sreenath, Taduru;Haruyama, Naoto;Honeycutt, Cherlita;Terse, Anita;Cho, Andrew;Kohler, Thomas;Mueller, Ralph;Goldberg, Michel;Kulkarni, Ashok B.
• 通讯作者: Kulkarni, Ashok B.

象牙質形成における Dentin sialoprotein と Dentin phosphoprotein の機能解析

牙本质唾液蛋白和牙本质磷蛋白在牙本质形成中的功能分析

• 发表时间: 2009
• 作者: Kubo T;Doi K;Hayashi K;Morita K;Matsuura A;Teixeira RE;Akagawa Y.;鈴木茂樹
• 通讯作者: 鈴木茂樹

Synergistic roles of amelogenin and ameloblastin.

• DOI: 10.1177/0022034509334749
• 发表时间: 2009-04
• 期刊: Journal of dental research
• 影响因子: 7.6
• 作者: Hatakeyama J;Fukumoto S;Nakamura T;Haruyama N;Suzuki S;Hatakeyama Y;Shum L;Gibson CW;Yamada Y;Kulkarni AB
• 通讯作者: Kulkarni AB