Hydrated ionic liquid for dissolution and refolding of proteins

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Physicochemical property of hydrated ionic liquid which is composed by cholinium cation ([ch]) and phosphate anion ([dhp]) has been analyzed with changing the water contents. The water molecules were interacted with ions strongly and existed as bound water. Proteins and nucleobase pairs were dissolved in hydrated [ch][dhp] with remained the higher ordered structure. Furthermore, the activity of dissolved biomolecules in hydrated [ch][dhp] were observed by spectroscopically and electrochemically. Furthermore, thermal stability and long term stability of the responses has been improved in hydrated [ch][dhp].