Search for metastasis-related proteins in colorectal cancer by proteome analysis

由于转移是结直肠癌最重要的预后因素之一，因此非常需要开发新的诊断和预防转移的方法。然而，转移表型的分子机制尚未得到很好的阐明。在这方面，我们寻找转移相关的蛋白质在结直肠癌中，通过分析蛋白质的差异表达的原发性和转移灶来源的结直肠肿瘤细胞使用蛋白质组学为基础的研究。为了验证这些候选蛋白，我们分析了这些蛋白在组织芯片中的表达谱。因此，Rho GDP解离抑制剂（Rho GDI）被鉴定为结肠癌患者中的转移相关蛋白。因此，Rho GDI可用于针对结肠癌转移的诊断或预防靶标。

Since metastasis is one of the most important prognostic factors in colorectal cancer, development of novel diagnostic and preventive methods for metastasis is highly desirable. However, the molecular mechanism of the metastatic phenotype has not been well elucidated. In this regard, we searched for metastasis-related proteins in colorectal cancer by analyzing the differential expression of proteins between primary and metastasis focus-derived colorectal tumor cells using a proteomics-based study. In order to validate these candidate proteins, we analyzed the expression profile of these proteins in tissue microarray. As a result, Rho GDP-dissociation inhibitor (Rho GDI) was identified as a metastasis-related protein in colon cancer patients. Consequently, Rho GDI may be useful for a diagnostic or preventive target against colon cancer metastasis.