Function of Z-DNA structure in Nrf2-dependent gene activation

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Left-handed double helix of DNA, called Z-DNA, is an alternative structure of DNA and appears to have many diverse roles in nuclear processes such as transcriptional regulation and nucleosome positioning. In this study, I have developed a method for in vivo detection of Z-DNA formation and show Nrf2 activation is associated with Z-DNA formation in the HO-1 gene promoter. The results implicate Nrf2-dependent Z-DNA formation in HO-1 gene transcriptional activation and suggestthe involvement of BRG1 in Z-DNA formation. Human HO-1 gene promoter might be a good model system to understand Z-DNA formation and how Z-DNA forming sequences regulate geneexpression.