Novel nucleic acids nanobiotechnology integrated with materials science

新型核酸纳米生物技术与材料科学相结合

• 批准号: 16200034
• 负责人: MARUYAMA Atsushi
• 金额: $ 30.62万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16200034/
• 关键词: Polycations; Comb-type copolymers; Polyelectrolytes; DNA; Hybridization; DNA nanomachines; 核酸シャペロン; グアニジノ基; 鎖交換反応; 核酸; 核酸鎖交換反応; ナノマシン; 核酸ナノテクノロジー; カチオン性共重合体; 遺伝子診断

1. Water soluble polyelectrolyte complex between cationic comb-type copolymer and nucleic acid We have been interested in the soluble interpolyelectrolyte complexes(IPEC)between DNA and the cationic comb-type copolymers, poly(L-lysine)-graft-dextran(PLL-g-Dex), consisting of a polylysine backbone and abundant hydrophilic graft chains of dextran as a model of nucleic acid-binding proteins. The copolymer forms a totally soluble IPEC in which the DNA coil-globule structural transition, i.e. compaction, is suppressed. The soluble nature of the complex enables us to spectroscopically monitor the effect of highly basic polymers on DNA hybridization. We found that an electrostatically equivalent amount of the comb-type copolymers increased stability of a double-stranded (ds)DNA and a triple-stranded DNA under physiological relevant conditions. Furthermore, we found that the copolymers stimulated the strand-exchange reaction between a ds DNA and its complementary single-stranded (ss)DNA, and e … More xhibit nucleic acid chaperoning activity.2. Acceleration of DNA hybridization by cationic comb-type copolymer Nucleic acid hybridization is a pivotal process in gene expression, replication and recombination in vivo. For biotechnology purposes, however, an increase in hybridization rate even at low DNA concentration is particularly important. Poly(L-lysine)-graft-dextran at nanomolar concentration significantly accelerated DNA-hybridization rate over 200-fold under physiologically relevant ionic conditions.3. Cationic comb-type copolymers for boosting DNA-fueled nanomachines For the better applications and developments of DNA nanomachines, their responding kinetics, output, and sequence-selectivity need to be improved. Furthermore, the DNA nanomachines currently have several limitations in operating conditions. In this study, we showed that poly(L-lysine)-graft-dextran produces the robust and quick responses of DNA nanomachines under moderate conditions including physiologically relevant conditions even at very low strand concentrations(nanomoles per liter range)through hybrid stabilization and DNA strand exchange acceleration. Less

1.阳离子梳型共聚物与核酸的水溶性嵌段复合物我们对DNA与阳离子梳型共聚物聚（L-赖氨酸）-葡聚糖接枝物（PLL-g-Dex）之间的可溶性嵌段复合物（IPEC）作为核酸结合蛋白的模型产生了兴趣。共聚物形成完全可溶的IPEC，其中DNA螺旋-小球结构转变，即压实，被抑制。复合物的可溶性使我们能够用光谱法监测高碱性聚合物对DNA杂交的影响。我们发现，静电当量的梳型共聚物增加了双链（ds）DNA和三链DNA在生理相关条件下的稳定性。此外，我们还发现共聚物能促进dsDNA与其互补单链DNA之间的链交换反应， ...更多信息 表达核酸伴侣活性。阳离子梳型共聚物加速DNA杂交核酸杂交是基因表达、复制和重组的关键过程。然而，对于生物技术目的，即使在低DNA浓度下杂交率的增加也是特别重要的。在生理相关离子条件下，纳摩尔浓度的聚L-赖氨酸-葡聚糖接枝物显著加快DNA杂交速率200倍以上.为了更好地应用和发展DNA纳米机器，需要改进其响应动力学、输出和序列选择性。此外，DNA纳米机器目前在操作条件方面有几个限制。在这项研究中，我们表明，聚（L-赖氨酸）-接枝-葡聚糖通过杂交稳定化和DNA链交换加速，在包括生理相关条件在内的温和条件下，即使在非常低的链浓度（纳摩尔/升范围）下，也能产生DNA纳米机器的稳健和快速反应。少

项目成果

Synergistic stabilization of nucleic acid assembly by oligo-N3'->P5' phosphoramidate modification and comb-type cationic copolymer

寡聚-N3->P5氨基磷酸酯修饰和梳型阳离子共聚物对核酸组装的协同稳定作用

• 发表时间: 2005
• 期刊: J.Am.Chem.Soc 127
• 作者: Takemoto M;et al.;H.Torigoe et al.
• 通讯作者: H.Torigoe et al.

人工核酸シャペロンを利用したDNAナノマシンの制御

使用人工核酸伴侣控制 DNA 纳米机器

• 发表时间: 2006
• 作者: 崔成 源;他
• 通讯作者: 他

DNA Nanomachine Switching Improved by Cationic Comb-Type Copolymer

• DOI: 10.1002/masy.200750352
• 发表时间: 2007-04
• 期刊: Macromolecular Symposia
• 作者: S. Choi;Naoki Makita;Arihiro Kano;A. Yamayoshi;T. Akaike;A. Maruyama

PH-sensing nano-crystals of carbonate apatite: Effects on intracellular delivery and release of DNA for efficient expression into mammalian cells

• DOI: 10.1016/j.gene.2006.02.028
• 发表时间: 2006-07-05
• 期刊: GENE
• 影响因子: 3.5
• 作者: Chowdhury, E. H.;Maruyama, A.;Akaike, T.
• 通讯作者: Akaike, T.

pH-sensing nano-crystals of carbonate apatite : Effects on intracellular deliverty and release of DNA for efficient expression into mammalian cells

pH 传感碳酸盐磷灰石纳米晶体：对细胞内 DNA 递送和释放的影响，以有效表达到哺乳动物细胞中

• 发表时间: 2006
• 期刊: Gene 367
• 作者: Itsuki;Ajioka;長澤榮治;E.H.Chowdhury et al.
• 通讯作者: E.H.Chowdhury et al.