Identification of novel mechanisms of Morbillivirus to interact withhost cells

核蛋白(N)是莫氏病毒核糖核蛋白复合体(RNP)的主要组成部分，包裹着它们的非片段负链病毒基因组RNA(VRNA)。病毒RNA依赖的RNA聚合酶复合体转录vRNA。麻疹病毒(MV)等麻疹病毒很容易形成持续性感染，并形成核内包涵体。N也是病毒核内包涵体的主要成分。我们发现，在建立MV持久性的过程中，MV的融合活性降低。然后，我们证明了N的亚细胞定位受其磷酸化的调节。最后，我们建立了由CAG启动子(N-Tg)表达N的转基因小鼠。N-TG在心肌和骨骼肌中形成包涵体。在这些肌肉中，N-TG显示肌肉萎缩。

The nucleoprotein (N) is the major component of the Morvillivirusribonucleoprotein complex (RNP) and encapsidates their non-segmented negative-stranded viral genome RNA (vRNA). The viral RNA dependent RNA polymerase complex transcribes vRNA. Morbillivirus, such as measles virus (MV), establishes a persistent infection easily and forms intranuclear inclusions. N is also the major component of this viral intranuclear inclusion. We found that fusion activity of MV was reduced during the establishment of MV persistence. Then, we demonstrated that the subcellular localization of N was regulated by its phosphorylation. Finally, we established transgenic mice expressing N by a CAG promoter (N-Tg). N-Tg formed inclusion bodies in cardiac muscle and skeletal muscle. In these muscles, N-Tg showed muscle atrophy.