Effects of chronic methamphetamine administration on change of dopamine transporter protein expression in striatum and nucleus accumbens, and the mechanisms of methamphetamine induced psychosis

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Observing the changes in the density of DA receptor and dopamine transporter expression in rat striatum and nucleus accumbens by chronic methamphetamine (MAP) administration. The real-time PCR and RT-PCR methods, and western-blotting method were used for measurement of m-RNA and protein, respectively. As a result, the DAT-mRNA expression level and the DAT-protein expression level of a chronic MAP administration group were decreased significantly. Decrease in DAT density inhibited the reuptake of DA, which was increased by MAP in the extracellular space. Further, long-term stimulatory effect by DAT inhibition will lead to dependent formation by MAP.