The study of Migration of Bone Marrow Derived Microglia (BMDM) to the Cerebellum in Adult Mice induced by Cranial Irradiation.

颅内辐射诱导成年小鼠骨髓衍生小胶质细胞 (BMDM) 向小脑迁移的研究。

• 批准号: 23791395
• 负责人: KAMINUMA Takuya
• 金额: $ 2.58万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791395/
• 关键词: 放射線; 神経細胞; ミクログリア; 国際情報交流

Recent studies have shown that adult microglia derived from primitive myeloid progenitors arising prior to embryonic day 8, and postnatal hematopoietic progenitors little contribute to microglia homeostasis in the brain of adult mice. On the other hand, some studies have suggested that “whole body irradiation" induced migration of bone marrow cells like microglia (BMDM) to the brain. Donor bone marrow cells (BM) were obtained from transgenic mice expressing the enhanced GFP. The GFP expressing BM cells were transplanted into adult mice. Four weeks after the BM transplant, one group received cranial irradiation of 13 Gy (n = 5), while another group was observed without irradiation (n = 5). Eight weeks after the cranial irradiation, 50-mm vibratome sections of the brain were obtained. In the sections of the group received whole body and cranial irradiation, GFP-expressing cells were present diffusely throughout the cerebellum. In contrast, only a few GFP-expressing cells were detected in the group received just whole body irradiation. Our results suggest that cranial irradiation, not whole body irradiation, induces migration of peripheral BMDM to the brain in adult mice.

最近的研究表明，成年小胶质细胞来源于胚胎第8天之前产生的原始髓样祖细胞，和出生后的造血祖细胞对成年小鼠脑中小胶质细胞的稳态几乎没有贡献。另一方面，一些研究表明，“全身照射”诱导骨髓细胞如小胶质细胞（BMDM）迁移到大脑。供体骨髓细胞（BM）从表达增强的GFP的转基因小鼠获得。将表达GFP的BM细胞移植到成年小鼠中。骨髓移植后4周，一组接受13戈伊的颅脑照射（n = 5），另一组不接受照射（n = 5）。头部照射后8周，获得50 mm的脑振动切片。在接受全身和颅骨照射的组的切片中，GFP表达细胞弥漫性地存在于整个小脑中。相比之下，仅在接受全身照射的组中检测到少数GFP表达细胞。我们的结果表明，头部照射而不是全身照射会诱导成年小鼠外周BMDM迁移到大脑。

项目成果

頭部へのX線照射によるBone Marrow Derived Microglia(BMDM)の誘導

通过头部 X 射线照射诱导骨髓衍生小胶质细胞 (BMDM)

• 发表时间: 2013
• 作者: Fukumitsu N;Oshiro Y;他7名;小此木 範之
• 通讯作者: 小此木 範之

頭部へのX線照射によるBone Marrow Derived Microglia (BMDM)の誘導

通过头部 X 射线照射诱导骨髓衍生小胶质细胞 (BMDM)

• 发表时间: 2013
• 作者: 小此木範之;神沼拓也
• 通讯作者: 神沼拓也

Cranial Irradiation Induces Migration of Bone Marrow Derived Microglia (BMDM) to the Cerebellum in Adult C57BL/6 Mice.

颅脑辐射诱导成年 C57BL/6 小鼠骨髓源性小胶质细胞 (BMDM) 迁移至小脑。

• 发表时间: 2012
• 作者: Okonogi N;Nakamura K;Suzuki Y;Suto N;Kaminuma T;Nakano T and Hirai H.
• 通讯作者: Nakano T and Hirai H.

Cranial irradiation induces migration of bone marrow derived cells to the cerebellum and their differentiation into microglia in adult mice

颅脑照射诱导成年小鼠骨髓来源的细胞迁移至小脑并分化为小胶质细胞

• 发表时间: 2013
• 作者: N.Okonogi;T.kaminuma
• 通讯作者: T.kaminuma

頭部への X 線照射による Bone Marrow Derived Microglia (BMDM) の誘導

通过头部 X 射线照射诱导骨髓衍生小胶质细胞 (BMDM)

• 发表时间: 2012
• 作者: 小此木範之;神沼拓也
• 通讯作者: 神沼拓也