Change of nano and microstructure of cellulose by surfactant treatment and its effect on promotion of diffusion and adsorption of cellulase

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In this study, it was shown that treatment of partially delignified lignocellulose with surfactant significantly enhanced cellulase's accessibility to cellulose fibrils through the changes of cellulose microfibril structure. Since a feasible delignification method is a key for the practical application of this method, the combination of surfactant treatment with biological delignification using a white-rot fungi or alkaline treatment was examined. As a result, the combination of the surfactant treatment with the alkaline treatment allowed the experimental condition to be milder. Hence, a semi-batch saccahrification and fermentation system with the surfactant treatment was proposed. It was found that the saccharfication efficiency was increased during the semi-batch reaction. Moreover, it was expected that the proposed concept can be practical due to its simplicity.