Fundamental Engineering Study on Effects of Stress on Brain Metabolism

众所周知，许多人在日常生活中因压力而患上疾病。为了克服这种情况，迄今为止在各个领域已经开展了许多关于应激反应的研究。作为工程方法的第一步，研究了通过血脑屏障和脑能量代谢的质量传递的简单方法。在体脉冲注射实验技术的发展建立了一种简单的实验技术，通过将放射性同位素示踪剂注入大鼠颈动脉，测量颈静脉流出液浓度的变化，来测量通过血脑屏障的质量传递和脑代谢。所用的示踪剂包括葡萄糖，葡萄糖容易透过血脑屏障，并在脑中部分降解。简化的数学模型在一级近似下，提出了描述脑中葡萄糖转运和代谢的数学模型，并用于理解如上所述导出的实验数据。与文献值相比，所得的总体参数是合理的.在体灌注脉冲注射法脑灌注和脉冲注射技术的成功建立，为有效积累化学物质在血脑屏障的传质数据提供了可能。

It is known that many people are suffering from the diseases caused by stress in their daily life. In order to overcome this situation, many researches on the stress responses have been carried out so far in various areas. As the first step towards the engineering approach for this purpose, the simple methodology for evaluating the mass transfer through blood brain barrier and brain energy metabolism was investigated.1. Development of experimental techniques of in vivo pulse injectionA simple experimental technique was developed to measure mass transfer through blood brain barrier and brain metabolism, where radio isotope tracers were injected into carotid artery of rat and the effluent concentration changes were measured at cervical vein. The tracers used involved glucose which easily permeate the blood brain barrier and partly degraded in the brain.2. Simplified mathematical modelAt the first approximation, the mathematical model describing glucose transport and metabolism in the brain was proposed and used for understanding the experimental data derived as above-mentioned. The resultant overall parameters were rational as compared to those in the literature.3. In vivo perfusion pulse injection methodBrain perfusion and pulse injection techniques were successfully established, which made it possible to accumulate the data on BBB mass transfer of chemicals efficiently.