Fundamental Engineering Study on Effects of Stress on Brain Metabolism

众所周知，许多人在日常生活中都患有由压力引起的疾病。为了克服这种情况，目前在各个领域开展了许多关于应力反应的研究。作为实现这一目的的工程方法的第一步，研究了评估血脑屏障传质和脑能代谢的简单方法。建立了一种简便的测定血脑屏障传质和脑代谢的实验方法，将放射性同位素示踪剂注入大鼠颈动脉，测量颈静脉流出物浓度变化。所使用的示踪剂含有葡萄糖，它很容易穿过血脑屏障，并在大脑中部分降解。在第一个近似中，我们提出了描述葡萄糖在大脑中运输和代谢的数学模型，并用于理解上述实验数据。所得的总体参数与文献中的参数相比是合理的。体内灌注脉冲注射方法成功建立了脑灌注和脉冲注射技术，为有效积累化学物质血脑屏障传质数据提供了可能。

It is known that many people are suffering from the diseases caused by stress in their daily life. In order to overcome this situation, many researches on the stress responses have been carried out so far in various areas. As the first step towards the engineering approach for this purpose, the simple methodology for evaluating the mass transfer through blood brain barrier and brain energy metabolism was investigated.1. Development of experimental techniques of in vivo pulse injectionA simple experimental technique was developed to measure mass transfer through blood brain barrier and brain metabolism, where radio isotope tracers were injected into carotid artery of rat and the effluent concentration changes were measured at cervical vein. The tracers used involved glucose which easily permeate the blood brain barrier and partly degraded in the brain.2. Simplified mathematical modelAt the first approximation, the mathematical model describing glucose transport and metabolism in the brain was proposed and used for understanding the experimental data derived as above-mentioned. The resultant overall parameters were rational as compared to those in the literature.3. In vivo perfusion pulse injection methodBrain perfusion and pulse injection techniques were successfully established, which made it possible to accumulate the data on BBB mass transfer of chemicals efficiently.