Induction of spermatogenic cells from human multipotent stem cell by microinjection into the mouse testis

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EGFP-tagged Muse cells from human mesenchymal cells were prepared by established method. Thus prepared EGFP-Muse cells were microinjected into the testes (testicular seminiferous tubules) of W/Wv mutant male mice lacking spermatogenic stem cells, and we found the following results. Enough amount of live Muse cells were obtained even after long time journey for several hours. Muse cells prepared caused cell-cell aggregation when they were placed in the conditioned medium containing albumin for microinjection, but the microinjection efficiency rate increased when they were microinjected using medium lacking albumin. However, 3 month later, EGFP-Muse cell signal was not recognized at all in the injected testes, indicating that Muse cells in the transplanted medium in this study could not develop to the spermatogenic cells.