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Molecular and cell biological study on the expression of germ cell function.

生殖细胞功能表达的分子和细胞生物学研究。

基本信息

批准号：
09670021
负责人：
TOSHIMORI Kiyotaka
金额：
$ 1.66万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

[Transport of acrosome materials] The fonnation of the acrosome was hamperd when the vesicle transport inhibitor, breferdin, was added to the culture medium (Tanji et al. (1998) J Electron Microsc, 47 ; 161). [Modification of acrin 1 (MN7=90K) during epididymal maturation] Acrin 1 changed its localization accompanying the morphological change during epididymal maturation (Yoshinaga et at. (1998) Cell Tiss Res, 292 ; 427.). [Function of equatorin (MN9=48K)] The equatorin remained at the equatorial segment until sperm reached to the perivitelline space, although some amount of the equatorin was released during the acrosome reaction. Sperm could not penetrate into the egg, and remained in the perivitelline space under in vitro fertilization (IVF) condition in the presence of anti-equatorin antibody (mMN9). The cortical granules could not be released from the egg. Both pronuclear formation and 2-cell formation were inhibited significantly. Thus, the equatorin is found to be an essential molecule for sperm-egg membrane fusion. (Toshimori et al. (1998) Biol Reprod, 59 ; 22.). [Function of acrin 1 and acrin 2 (MC41=165K)] Acrin 1 was required for the acrosome reaction, while acrin 2 was required for a certain process during after acrosome reaction but before penetration through the zona pellucida. (Saxena et al. (1999) J Reprod Fert, in press.) (Function of acrin 3 (MC1O1=155K)] Acrin 3 was suggested to be related to the gamete fusion, but the detail of the mechanism remained unclear. [Cloning of MC31 antigen (26-35K)] MC31 was highly homologous to the CE9 molecule of rat sperm, which is a molecule of immunoglobulin superfamily. [Review] The maturation of sperm antigenic molecules was reviewed. (Toshimori K (1998) Cell Tissue Res, 293 ; 177.).

[顶体物质的运输]当向培养基中加入囊泡运输抑制剂breferdin时，阻碍了顶体的形成（Tanji等，（1998）J Electron Microsc，47 ; 161）。[在附睾成熟过程中Acrin 1（MN 7 = 90 K）的修饰] Acrin 1在附睾成熟过程中伴随形态学变化而改变其定位（Yoshinaga et at.（1998）Cell Tiss Res，292 ; 427.）。[赤道素的作用（MN_（9）= 48 K）]赤道素在精子到达卵周间隙之前一直停留在赤道段，尽管在顶体反应过程中有一定量的赤道素释放。在体外受精（IVF）条件下，在存在抗赤道素抗体（mMN 9）的情况下，精子不能穿透卵子，并留在卵黄周隙中。皮质颗粒不能从卵中释放出来。原核形成和2-细胞形成均受到明显抑制。因此，赤道素被认为是精卵膜融合所必需的分子。（Toshimori等人（1998）Biol Reprod，59 ; 22.）。[acrin 1和acrin 2的功能（MC 41 = 165 K）] Acrin 1是顶体反应所必需的，而acrin 2是顶体反应后但穿透透明质膜之前的某个过程所必需的。（Saxena等人（1999）J Reprod Fert，出版中。（Acrin 3的功能（MC 1 O 1 = 155 K））Acrin 3被认为与配子融合有关，但其具体机制尚不清楚。[MC 31抗原的克隆（26- 35 K）] MC 31与大鼠精子免疫球蛋白超家族分子CE 9高度同源。[综述]对精子抗原分子的成熟进行了综述。（Toshimori K（1998）Cell Tissue Res，293 ; 177.）。