ANALYSES OF SPERM-EGG INTERACTION AND MECHANISM OF INFERTILITY USING INFERTILITY RELATED GENE KNOCKOUT MOUSE

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I.Functional expression and molecular modification of a sperm flagellar protein MC31/CE91)We have clarified; 1)behavior and rote of MC31 /CE9 during sperm-egg interaction, especially during the acrosome reaction in the rat (Saxena and Toshimori (2004) Biol Reprod.); molecular size was reduced during epididymal transit and acrosome reaction, MC31 was migrated from tail region to head region during acrosme reaction and involved in sperm-egg attachment. 2)We have reviewed role of immunoglobulin superfamily protein (Toshimori, (2003) Chiba Medical Journal ; Toshimori, (2003) Kluwer publisher)II.Functional expression and behavior of an oocyte-activation related protein MN131)We have clarified behavior and roles of MN13 molecule during fertilization and early development (Manandhar and Toshimori., 2003, Biol Reprod); MN13 was involved in oocyte activatio, migrating to the pole of chromosome spindle during division of metaphase II.III.Functional expression of sperm specific protein MN7 (acrin … More 1)Papers published in relation to new finding as to spergen-1 and spergen-2 molecules (Doiguchi et al., 2002, Biol Reprod ; Iida et al (2003) Blot Reprod)IV.GOPC and mGCL.We have clarified the relationship between the etiology of infertility and malformation of sperm head and tail. GOPC -/-male mouse was found. to be an animal model of glozoospermia in the human infertility syndrome (Yao et al., 2002, PRONAS USA). mGCL -/-male moose vas an infertility model due to multi-phenotype of sperm head and tails (Kimura et al., 2003, Mot Cell Biol. We also reviewed the formation and organization of sperm head in relation to GOPC and mgGCL (Toshimori and Ito (2003) Arch Histol Cytol).V.Relationship between carnitine and sperm maturation and infertility or obstructive azoospermiaTwo papers are now under submission ; one is for a new finding of carnitine transporter OCTN2 specifically localized in the pancreatic A cells (Kai et al.) and the other is for a relationship between OCTN2 localization and obstructive infertility found in the OCTN2 mutant (JVS) mouse epididymis (Yakushiji et al). Less