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ANALYSES OF SPERM-EGG INTERACTION AND MECHANISM OF INFERTILITY USING INFERTILITY RELATED GENE KNOCKOUT MOUSE

利用不孕相关基因敲除小鼠分析精卵相互作用及不孕机制

基本信息

批准号：
14570019
负责人：
TOSHIMORI Kiyotaka
金额：
$ 2.62万
依托单位：
CHIBA UNIVERSITY (2003)宮崎医科大学 (2002)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

I.Functional expression and molecular modification of a sperm flagellar protein MC31/CE91)We have clarified; 1)behavior and rote of MC31 /CE9 during sperm-egg interaction, especially during the acrosome reaction in the rat (Saxena and Toshimori (2004) Biol Reprod.); molecular size was reduced during epididymal transit and acrosome reaction, MC31 was migrated from tail region to head region during acrosme reaction and involved in sperm-egg attachment. 2)We have reviewed role of immunoglobulin superfamily protein (Toshimori, (2003) Chiba Medical Journal ; Toshimori, (2003) Kluwer publisher)II.Functional expression and behavior of an oocyte-activation related protein MN131)We have clarified behavior and roles of MN13 molecule during fertilization and early development (Manandhar and Toshimori., 2003, Biol Reprod); MN13 was involved in oocyte activatio, migrating to the pole of chromosome spindle during division of metaphase II.III.Functional expression of sperm specific protein MN7 (acrin … More 1)Papers published in relation to new finding as to spergen-1 and spergen-2 molecules (Doiguchi et al., 2002, Biol Reprod ; Iida et al (2003) Blot Reprod)IV.GOPC and mGCL.We have clarified the relationship between the etiology of infertility and malformation of sperm head and tail. GOPC -/-male mouse was found. to be an animal model of glozoospermia in the human infertility syndrome (Yao et al., 2002, PRONAS USA). mGCL -/-male moose vas an infertility model due to multi-phenotype of sperm head and tails (Kimura et al., 2003, Mot Cell Biol. We also reviewed the formation and organization of sperm head in relation to GOPC and mgGCL (Toshimori and Ito (2003) Arch Histol Cytol).V.Relationship between carnitine and sperm maturation and infertility or obstructive azoospermiaTwo papers are now under submission ; one is for a new finding of carnitine transporter OCTN2 specifically localized in the pancreatic A cells (Kai et al.) and the other is for a relationship between OCTN2 localization and obstructive infertility found in the OCTN2 mutant (JVS) mouse epididymis (Yakushiji et al). Less

I.精子鞭毛蛋白MC 31/CE 91的功能表达和分子修饰）我们已经阐明：1）在大鼠中精子-卵子相互作用期间，特别是在顶体反应期间，MC 31/CE 9的行为和作用（Saxena和Toshimori（2004）Biol Reprod.）; MC 31在附睾转运和顶体反应过程中分子量减小，在顶体反应过程中从尾区迁移到头区，参与精卵附着。2）我们已经综述了免疫球蛋白超家族蛋白的作用（Toshimori，（2003）千叶医学杂志; Toshimori，（2003）Kluwer出版商）II.卵母细胞活化相关蛋白MN 131的功能表达和行为）我们已经阐明了MN 13分子在受精和早期发育过程中的行为和作用（Manandhar和Toshimori.，2003，Biol Reprod）; MN 13参与卵母细胞激活，在中期分裂时迁移到染色体纺锤体的极点II.III.精子特异蛋白MN 7（acrin）的功能表达 ...更多信息 1)发表的关于精子原-1和精子原-2分子的新发现的论文（Doiguchi等人，2002，Biol Reprod ; Iida et al（2003）Blot Reprod）IV.GOPC和mGCL。发现GOPC -/-雄性小鼠是人类不育综合征中的球形精子症的动物模型（Yao等人，2002，PRONAS USA）。mGCL -/-雄性驼鹿由于精子头部和尾部的多表型而成为不育模型（Kimura等人，2003，Mot Cell Biol。我们还综述了与GOPC和mgGCL相关的精子头部的形成和组织（Toshimori和Ito（2003）Arch Hirosis Cytol）。另一个是OCTN 2定位和在OCTN 2突变（JVS）小鼠附睾中发现的阻塞性不育之间的关系（Yakushiji等）。少