权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

MOLECULAR AND CELLULAR STUDIES ON THE FUNCTIONAL EXPRESSION OF SPERM SPECIFIC PROTEIN AND ON THE CARNITINE-DEPENDENT SPERM MATURATION

精子特异性蛋白功能表达和肉碱依赖性精子成熟的分子和细胞研究

基本信息

批准号：
12670022
负责人：
TOSHIMORI Kiyotaka
金额：
$ 2.62万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

I. Functional expression of sperm specific proteins, acrin 1 (MN7), acrin 2 (MC41), acrin 3 (MG101), equatorin (MN9)1) acrin 1 (MN7) : We clarified rganization and modification during sperm maturation in the gunea pig (Yoshinaga et1 al., 2000) and human (Oh-oka et al., 2001).2) acrin 2 (MC41) : We clarified 1) reorganization within the acrosome, during epididymal maturation (Yoshinaga et al., 2001) and 2) that acrin 2 involved in firm binding to zona pellucida after the acrosome reaction (Tanii et al., 2001)3) acrin 3 (MC101) : We have analyzed that acrin 3 is functionally involved in the process after the acrosome reaction but before penetration into the zona pellucida (Saxena et al., 2000).4) equatorin (MH9) : We have clarified 1) that equatorin is involved in sperm-egg fusion process by translocating to the plasma membrane at the equatorial segment after the acrosome reaction (Manandhar and Toshimori, 2001) and 2) that equatrorin also functions under in vivo condition (Yoshinaga et al., 2001).II. Functional expression and molecular modification of sperm flagellar proteins MC31/CE9 and basigin1) We have clarified 1) MC31/GE9, expression at gene level using in situ hybridization (Wakayama et al., 2000), and 2) mouse basigin is involved in binding to zona pellucida after translocating to the anterior head region (Saxena et al., 2002). 3) We have reviewed the relationship between immunoglobulin superfamily protein and fertilization (Toshimori et al., 2000).III. Relationship between carnitine and sperm maturation/infertility (obstructive azoospermia)We made two specific antisera (H256 and K266), by which we are accumulating the expression of carnitine transporter in the testis, kidney and pancreas.

I.精子特异性蛋白质acrin 1（MN 7）、acrin 2（MC 41）、acrin 3（MG 101）、equatorin（MN 9）1）acrin 1（MN 7）的功能表达：我们阐明了gunea猪精子成熟过程中的组织和修饰（Yoshinaga等，2000）和人（Oh-oka等，2）acrin 2（MC 41）：我们阐明了1）在附睾成熟期间，顶体内的重组（Yoshinaga等人，2001）和2）acrin 2参与顶体反应后与透明质酸的牢固结合（Tanii等人，2001）3）acrin 3（MC 101）：我们已经分析了acrin 3在顶体反应之后但在渗透到透明细胞之前在功能上参与该过程（Saxena等人，4）赤道蛋白（MH 9）：我们已经阐明1）赤道蛋白通过在顶体反应后易位至赤道段处的质膜而参与精卵融合过程（Manandhar和Toshimori，2001）和2）赤道蛋白也在体内条件下起作用（Yoshinaga等人，二.精子鞭毛蛋白MC 31/CE 9和basigin的功能表达和分子修饰1）我们已经阐明了1）MC 31/GE 9，使用原位杂交在基因水平上的表达（和歌山等人，2000），和2）小鼠basigin在易位到前头部区域后参与结合透明质酸（Saxena等人，2002年）。3)我们已经综述了免疫球蛋白超家族蛋白与受精之间的关系（Toshimori et al.，三.肉毒碱与精子成熟和不育（梗阻性无精子症）的关系我们制备了两种特异性抗血清（H256和K266），用它们积累了肉毒碱转运体在睾丸、肾脏和胰腺中的表达。