Detection of HMGB1 in acetaminophen-induced liver injury model and its application for forensic diagnosis

对乙酰氨基酚肝损伤模型中HMGB1的检测及其在法医学诊断中的应用

• 批准号: 23659370
• 负责人: ISHIDA Yuko
• 金额: $ 2.41万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659370/
• 关键词: HMGB1; アセトアミノフェン; 法医診断学

We examined HMGB1 expression in acetaminophen (APAP)-induced liver injury. mRNA expression of HMGB1 was enhanced in the liver of mice injected with APAP. Although HMGB1 protein was detected in hepatocyte nuclei of normal liver, it was observed in cytoplasm of hepatocytes after APAP challenge. The detection of HMGB1 in the liver could be considered as valuable diagnostic tools for the postmortem diagnosis of APAP-induced liver injury.

我们检测了对乙酰氨基酚（APAP）诱导的肝损伤中HMGB1的表达。注射APAP的小鼠肝脏HMGB1 mRNA表达增强。HMGB1蛋白在正常肝细胞核中表达，而在APAP刺激后，HMGB1蛋白在肝细胞胞浆中表达。HMGB1在肝脏中的检测可以被认为是有价值的诊断工具，为APAP诱导的肝损伤的死后诊断。