Production of a novel diagnostic tool for bullous pemphigoid using cleavage site-specific antibodies.

大疱性类天疱疮（BP）是一种自身免疫性水疱性疾病。本研究的目的是阐明BP的发病机制，这可能有助于早期和准确的诊断。为了解决这个问题，我们的目的是发现BP患者的水疱液可以切割胶原XVII（COL17），这是BP的自身抗原。我们已经建立了一种方法来获得高度纯化的重组COL17，它实际上是由来自BP患者的水疱液在跨膜NC16A结构域内切割的。

Bullous pemphigoid(BP) is an autoimmune blistering disease. The aim of this study is to clarify the pathogenesis of BP, which may be useful for early and accurate diagnosis. To address this, we aimed at the finding that blister fluid of BP patients can cleave the collagen XVII(COL17) which is an autoantigen for BP. We have established a method to obtain highly purified recombinant COL17, which was actually cleaved by blister fluid from BP patients within the juxtamembranous NC16A domain.