Development of the novel compound which specifically scavenges singlet oxygen extra- and intracellularly.

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Dimethyl-hydropterin (DPH) was newly synthesized through the reaction with pterin and edaravone. The direct analysis of near-infrared luminescence using Raman spectroscopy proved that DPH quenched the photochemically generated singlet oxygen in a cell-free system. Further, the chemiluminescence assay revealed that DPH scavenged not only singlet oxygen but also superoxide generated in activated neutrophils. When singlet oxygen was generated photochemically in neuronal cells, cell death was induced. DPH prevented the singlet oxygen-induced cell death. And, when keratinocytes was weakly exposed to the ionized radiation, cell death was not observed but the DNA damage was observed. DPH attenuated the DNA damage, probably due to scavenging singlet oxygen.