Determination of the active reactive oxygen species that generated in cells during ischemia-reperfusion and selection of its specific scavenger.

探讨活性氧(ROS)与细胞损伤的关系。在ROS中，我们注意到单线态氧(SO)，但尚未引起太多注意。人白血病细胞系NB4、大鼠神经细胞系B50、小鼠神经细胞系MG6、人中性粒细胞和大鼠神经元。在NB4中，产生SO诱导的血管细胞损伤。在B50细胞中，细胞外以坏死方式诱导急性细胞死亡。在MG6中，细胞内通过自噬诱导亚急性细胞死亡。在中性粒细胞中，SO通过形成Net诱导细胞死亡。在神经元中，SO参与了谷氨酸诱导的细胞死亡。在上述所有实验中，SO都强烈参与了细胞损伤。因此，我们推测SO在ROS诱导的细胞损伤中是必不可少的。

The relation of reactive oxygen species(ROS) with cell injury was investigated. Among ROS, we noticed singlet oxygen(SO) that has not been much noticed yet. Human leukemia cell line NB4, rat neuronal cell line B50, mouse neuronal cell line MG6, human neutrophils and rat neuron were used. In NB4, produced SO induced vascular cell injury. In B50, extracellularly generated SO induced acute cell death by necrosis. In MG6, intracellularly generated SO induced subacute cell death by autophagy. In neutrophils, SO induced cell death with NETs formation. In neurons, SO was involved in cell death induced by glutamate. In above mentioned all experiments, SO was strongly involved in cell injury. Therefore, we speculated that SO is essential for cell injury induced by ROS.