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A new method for the estimation of cerebral oxygen metabolism

一种估算脑氧代谢的新方法

基本信息

批准号：
02454353
负责人：
ARAI Toshiyuki
金额：
$ 4.42万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454353/
关键词：
brain blood flow oxygen consumption water-18 oxygen-18 gas GC-MS 酸素安定同位元素人工血液

项目摘要

A new method for the estimation of regional cerebral blood flow (rCBF) and oxygen consumption (rCMRO_2) using a stable isotope of oxygen was developed and the validity of the method was estimated.1. The measurement of rCBF(1) The method using trifluoroiodomethane (CF_3I) : The CF_3I resolved in saline was infused to a rat. During infusion, the arterial blood samplings were performed several times, and just after infusion, the brain was extracted and divided into several portions. The concentration of CF_3I both in blood samples and cerebral tissues was measured using gas chromatograph-mass spectrometer equipped with a headspace sampler (GC-MS-HSS). The RCBF was calculated with a modified autoradiographic strategy. However, due to the evaporation of CF_3I during sampling, the calculated values were proved to be incorrect.(2) The method using water-18 (H_2^<18>O) : The saline including 50% water-18 was injected to a rat. Following the injection, the arterial blood was sampled several times, and 30 sec after the injection, the brain was extracted. The concentration of water-18 in blood and tissue samples was measured using GC-MS-HSS, and the RCBF was calculated with a KetySchmit method. The calculated values followed the changes in rCBF induced by hypo- and hyperventilation, and were coincident with the previously reported values.(3) The method using water-18 was applied to rat ischemic models to estimate the validity of this method. The results showed the manifest decreases in RCBF at the site of ischemia, which proved the validity of the method using water-18.2. The measurement of rCMRO_2After the 10 min inhalation of 50% oxygen-18 gas, the rat brain was extracted and the concentration of metabolic water-18 was measured using GC-MS-HSS. However, this method was proved not to be adequate for the measurement of rCMRO_2.

提出了一种利用氧的稳定同位素估算脑区域血流量（rCBF）和耗氧量（rcro_2）的新方法，并对该方法的有效性进行了评价。rCBF的测定(1)三氟碘甲烷（CF_3I）法：将CF_3I溶解于生理盐水中，输注于大鼠。在输注过程中，多次动脉采血，刚输注完毕，提取脑并分成几部分。采用顶空进样气相色谱-质谱联用仪（GC-MS-HSS）测定血液和脑组织中CF_3I的浓度。RCBF采用改进的放射自成像策略计算。然而，由于采样过程中CF_3I的蒸发，计算值被证明是不正确的。(2)水-18 （H_2^<18>）法：大鼠体内注射含50%水-18的生理盐水。注射后多次取动脉血，注射后30秒取脑。采用GC-MS-HSS法测定血液和组织样品中水-18的浓度，采用keytyschmit法计算RCBF。计算值遵循低通气和过度通气引起的rCBF变化，与先前报道的值一致。(3)将水-18法应用于大鼠缺血模型，评价该方法的有效性。结果显示缺血部位RCBF明显减少，证明了水-18.2方法的有效性。吸入50%的氧-18气体10 min后，提取大鼠脑，用GC-MS-HSS测定代谢水-18的浓度。然而，这种方法被证明不适合测量rcmoro_2。