Effect of periodontopathic factors on osteoblastic differentiation of rat bone-marrow cells cultured under simulated microgravity

本研究探讨牙周病因子对模拟微重力培养大鼠骨髓细胞成骨分化的影响。实验条件为模拟微重力环境，牙龈卟啉脂多糖（P-LPS）为牙周病因子。微重力作用下骨髓细胞成骨分化，骨相关基因表达增加，骨结节形成。这些标记物被P-LPS抑制。dna芯片分析显示caspase基因表达增加，caspase是一种与内质网应激相关的分子。提示P-LPS可能影响微重力条件下成骨细胞的分化。

This study investigated the effect of periodontopathic factors on osteoblastic differentiation of cultured rat bone-marrow cells under simulated microgravity. Experimental conditions were made up using 3D-clinostat for the simulated microgravity and lipopolysaccharide from P. gingivalis(P-LPS) for the periodontopathic factor. Osteoblastic differentiation of bone marrow cells was progressed by the microgravity, showing increases of bone-associated gene expression and bone-nodule formation in culture. These markers were inhibited by the addition of P-LPS. DNA-microarray analysis revealed an increase of gene expression of caspase, a molecule associated with endoplasmic reticulum stress. These results suggest that P-LPS may affect on osteoblastic differentiation under the microgravity condition.