Diagnostic approaches by a susceptibility determination based on gene polymorphism in Japanese periodontitis patients.

日本牙周炎患者基于基因多态性的敏感性测定的诊断方法。

• 批准号: 16209062
• 负责人: NAGATA Toshihiko
• 金额: $ 32.03万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209062/
• 关键词: periodontal diseases; gene polymorphism; disease susceptibility; aggressive periodontitis; gingival overgrowth; immunoglobulin; cytokines; invader method

Polymorphism analyses using the invader method were performed (case control studies). From 12 hospitals, 622 blood samples were collected : 172 aggressive periodontitis (AgP) and the 178 controls, and 147 chronic periodontitis (CP) and the 125 controls. When AgP and the controls was compared, significant differences were detected in FcaR56T/C and MMP-3(-1171)5A/6A(-/T). When CP and the controls was compared, significant differences were detected in IL-1(+4845)G/T. In these SNPs, there were no SNPs showing both differences concerning gene distribution and allele frequencies. Further examinations are necessary to clarify these points. On the other hand, several interesting results concerning SNPs were obtained from the investigator's laboratories as follows. 1) In gingival overgrowth patients, a2 integrin +807 polymorphism was found, indicating the +807C allele is one of the genetic risk factors for drug-induced gingival overgrowth. 2) The combination of stimulatory FcyIIA and inhibitory FcyRIIB genotypes may increase susceptibility to SLE and periodontitis in the Japanese population. 3) Salivary AST, ALT and LDH levels reflect inflammation and destruction of periodontal tissue, suggesting the clinical useful markers following periodontal therapy but IL-1A+4845 alleles may not influence clinical parameters. 4) The mutant (A115V) TNSALP gene produced the defective alkaline phosphatase enzyme and it could be recessively transmitted and be a disease-causing mutation of the adult-type hypophosphatasia 5) Mannnose-binding lectin (MBL) gene mutation and smoking would be involved in the excerbation of aggressive periodontitis, via gene-environmental interaction. (229 words)

使用入侵者方法进行多态性分析（病例对照研究）。从12家医院收集了622份血液样本：172例侵袭性牙周炎（AgP）和178例对照，147例慢性牙周炎（CP）和125例对照。当比较AgP和对照组时，在FcaR 56 T/C和MMP-3（-1171 5A/6A（-/T）中检测到显著差异。CP组与对照组比较，IL-1（+4845）G/T有显著性差异。在这些SNPs中，没有SNPs显示基因分布和等位基因频率的差异。有必要进一步检查以澄清这些问题。另一方面，从研究者的实验室获得了如下关于SNP的几个有趣的结果。1)在牙龈增生患者中发现整合素α 2基因+807位点多态性，提示+807C等位基因是药物性牙龈增生的遗传危险因素之一。2)刺激性Fc γ IIA和抑制性Fc γ RIIB基因型的组合可能增加日本人群对SLE和牙周炎的易感性。3)唾液AST、ALT和LDH水平反映牙周组织的炎症和破坏，提示牙周治疗后的临床有用的标志物，但IL-1A+4845等位基因可能不影响临床参数。4)TNSALP基因突变（A115 V）产生的碱性磷酸酶缺陷型可通过遗传途径传播，是成人型低磷酸酶症的致病突变。5）甘露糖结合凝集素（MBL）基因突变和吸烟可能通过基因-环境相互作用参与侵袭性牙周炎的发病。(229字）

项目成果

Interleukin-6 (IL-6)-373 A9T11 allele is associated with reduced susceptibility to chronic periodontitis in Japanese subjects and decreased serum IL-6 level

• DOI: 10.1111/j.1399-0039.2005.00347.x
• 发表时间: 2005-01-01
• 期刊: TISSUE ANTIGENS
• 作者: Komatsu, Y;Tai, H;Yoshie, H
• 通讯作者: Yoshie, H

Fos plays no role in apoptosis of epithelia in the mouse male accessory sex organs and uterus.

Fos 在小鼠雄性副性器官和子宫的上皮细胞凋亡中没有作用。

• 发表时间: 2005
• 期刊: Endocr J. 52(1)
• 作者: Oh EH;Hirose M;Takemura T;Yoshihara H;Ishizaki T;Imanaka Y.;Kuhara A
• 通讯作者: Kuhara A

The role of genetic polymorphism in periodontitis.

遗传多态性在牙周炎中的作用。

• 发表时间: 2007
• 期刊: Periodontology 2000 (印刷中)
• 作者: Mitsuo;Ochi;Yoshie H.
• 通讯作者: Yoshie H.

Focal adhesion kinase(FAK)mediates human leukocyte histocompatibility antigen(HLA) CLASS II-induced signaling in gingival fibroblasts.

粘斑激酶 (FAK) 介导人类白细胞组织相容性抗原 (HLA) II 类诱导的牙龈成纤维细胞信号传导。

• 发表时间: 2007
• 期刊: Journal of Periodontal Research (In press)
• 作者: 三井将;小林誠;ら;Shoji Nishio;Yuji Ohtsuki;Yasushi Uemura;Hideki Ohyama;Tian-Yi Liu;Hideki Ohyama;Yuka Okada;Yuji Ohtsuki;Yasushi Uemura;Hideki Ohyama;Tian-Yi Liu;Hideki Ohyama;Yuka Okada;Kazu Takeuchi-Hatanaka;Hideki Ohyama;Tian-Yi Liu;Yasushi Uemura;Sayuri Yoshizawa;Koji Yamanegi;Koji Yamanegi;脇本 栄子;中正 恵二;中正 恵二;Sayuri Yoshizawa;Koji Yamanegi;Koji Yamanegi;Sayuri Yoshizawa
• 通讯作者: Sayuri Yoshizawa

A novel polymorphism of FcaRI (CD89) associated with aggressive periodontitis.

FcaRI (CD89) 的一种新多态性与侵袭性牙周炎相关。

• 发表时间: 2004
• 期刊: Tissue Antigens 63(6)
• 作者: Zhao L;Ikeda Y;Stalling NR;Reuter AL;Beck L;Tobet SA;Parker KL;春山成子;Kaneko S
• 通讯作者: Kaneko S