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Molecular biology of pathological conditions and diagnosis in dental pulp disorder and periodontal disease.

牙髓疾病和牙周疾病的病理状况和诊断的分子生物学。

基本信息

批准号：
10307047
负责人：
NAGATA Toshihiko
金额：
$ 8.51万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10307047/
关键词：
dental pulp diseases periodontal diseases cytokine gene PCR LPS growth factors fibroblasts 転写因子免疫組織化学アポトーシスリポ多糖

项目摘要

(1) Summary concerning dental pulp diseases : Type I collagen and osteopontin were found in human dental pulp stones and osteopontin expressin was stimulated by retinoic acid in culturted dental pulp cells. When the localization of DNA fragment after laser irradiation was investigated, dental pulps were affected at the early stage of cavity preparation. Expression of VEGF was induced by inflammatory cytokines in cultured dental pulp cells, which was mediated through AP-1 activations.(2) Summary concerning periodontal diseases : RT-PCR analyses using inflammed gingival tissues of adult periodotitis revealed that high levels of P.gingivalis, T.denticola, and B.forsythus were found. Novel RT-PCR examination system using very little gingival tissues was established, in which over 20 mRNAs of inflammatory factors were detected from the periodontal lesions and semi-quantified the volume of the mRNAs. In cultured gingival fibroblasts, various proteins induced by IL-1 were transfered from predominant type to mature type. Adhesion molecules expressed by TNF-α were down-regulated by PGE_2 and I_2 in gingival epithelial cells. LPS form P.gingivalis inhibited osteoblastic differentiation of cultured rat calvaria cells. CD 14 was associated with calcium signal pathway stimulated by LPS.Expression of SPARC and OCIF was changed with the ageing of periodontal ligament cells and SPARC has an important role with the healing of periodontal tissues. In high glucose conditions, activity of cathepsin was inhibited in periodontal ligament cells. Phenytoin and Cyclosporin A were inhibited the expressions of MMP-1 and TIMP-1.

(1)关于牙髓疾病的综述：人牙髓结石中发现I型胶原和骨桥蛋白，在培养的牙髓细胞中维甲酸刺激骨桥蛋白表达。在研究激光照射后DNA片段的定位时，牙髓在造腔前期就受到了影响。在体外培养的牙髓细胞中，炎症因子通过激活AP-1介导VEGF的表达。(2)关于牙周病的综述：对成人牙周炎的炎症牙龈组织进行RT-PCR分析，发现高水平的P.gingivalis、T.denticola和B.forsythus。建立了利用极少量牙龈组织的新型RT-PCR检测系统，从牙周病变处检测到20多种炎症因子mrna，并对mrna的体积进行半量化。在培养的牙龈成纤维细胞中，IL-1诱导的多种蛋白从优势型向成熟型转移。PGE_2和I_2可下调牙龈上皮细胞中TNF-α表达的粘附分子。牙龈卟啉卟啉脂多糖抑制培养大鼠颅骨细胞成骨分化。cd14与LPS刺激的钙信号通路有关。随着牙周韧带细胞的老化，SPARC和OCIF的表达发生变化，SPARC在牙周组织的愈合中起着重要作用。在高糖条件下，牙周韧带细胞组织蛋白酶活性受到抑制。苯妥英和环孢素A抑制MMP-1和TIMP-1的表达。